Model Evaluation of the Microbial Metabolic Processes in a Hydrogen-Based Membrane Biofilm Reactor for Simultaneous...

The H₂-based membrane biofilm reactor (H₂-MBfR) has been acknowledged as a cost-effective microbial reduction technology for oxyanion removal from drinking water sources, but it remains unknown how the evolution of biofilm characteristics responds to the changing critical operating parameters of the H₂-MBfR for simultaneous bromate (BrO₃^-) and nitrate (NO₃^-) elimination. Therefore, an expanded multispecies model, applicable to mechanistically interpret the bromate-reducing bacteria (BRB)- and denitrifying bacteria (DNB)-dominated metabolic processes in the biofilm of the H₂-MBfR, was developed in this study. The model outputs indicate that (1) increased BrO₃^- loading facilitated the metabolism of BRB by increasing BRB fraction and BrO₃^- gradients in the biofilm, but had a marginal influence on NO₃^- reduction; (2) H₂ pressure of 0.04 MPa enabled the minimal loss of H₂ and the extension of the active region of BRB and DNB in the biofilm; (3) once the influent NO₃^- concentration was beyond 10 mg N/L, the fraction and activity of BRB significantly declined; (4) BRB was more tolerant than DNB for the acidic aquatic environment incurred by the CO₂ pressure over 0.02 MPa. The results corroborate that the degree of microbial competition for substrates and space in the biofilm was dependent on system operating parameters.