Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

araucan regulates butterfly wing iridescence by coordinating scale structure and pigmentation.

Proceedings. Biological sciences·2026
Same author

Disruption of <i>chrna5</i> blunts aversion and adaptive transcriptomic responses to nicotine and alcohol.

iScience·2026
Same author

UV wing patterns in saturniid moths: diversity and mechanisms.

The Journal of experimental biology·2025
Same author

Shared regulatory networks link vein positioning and eyespot ring formation in butterflies.

Communications biology·2025
Same author

A novel Hox gene promoter fuels the evolution of adaptive phenotypic plasticity in wing eyespots of satyrid butterflies.

Nature ecology & evolution·2025
Same author

<i>chrna3</i> Modulates Alcohol Response.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2025

Related Experiment Video

Updated: Aug 31, 2025

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc
15:59

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc

Published on: April 30, 2010

11.3K

Laser Microdissection-Mediated Isolation of Butterfly Wing Tissue for Spatial Transcriptomics.

Tirtha Das Banerjee1, Shen Tian1, Antόnia Monteiro1,2

  • 1Department of Biological Sciences, National University of Singapore, Singapore 117557, Singapore.

Methods and Protocols
|August 25, 2022
PubMed
Summary

This study presents a laser dissection protocol for RNA extraction from butterfly wings, enabling spatial transcriptomics without tissue embedding. This method aids in connecting gene expression to specific tissues during development.

Keywords:
Bicyclus anynanaRNAseqeyespotslaser microdissectionspatial transcriptomicswing sectors

More Related Videos

Laser Microdissection for Species-Agnostic Single-Tissue Applications
08:57

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Published on: March 31, 2022

2.3K
Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types
09:31

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Published on: May 10, 2016

9.4K

Related Experiment Videos

Last Updated: Aug 31, 2025

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc
15:59

Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc

Published on: April 30, 2010

11.3K
Laser Microdissection for Species-Agnostic Single-Tissue Applications
08:57

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Published on: March 31, 2022

2.3K
Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types
09:31

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Published on: May 10, 2016

9.4K

Area of Science:

  • Developmental biology
  • Genomics
  • Molecular biology

Background:

  • Connecting gene expression patterns to specific cells is crucial for understanding genotype-phenotype relationships.
  • Current methods like single-cell sequencing lack spatial context, while techniques like multiplex FISH require prior knowledge of target transcripts.
  • Spatial transcriptomics is essential for detailed developmental studies.

Purpose of the Study:

  • To detail a novel laser dissection protocol for total RNA extraction from butterfly larval and pupal wing tissues.
  • To provide a cost-effective and efficient method for unbiased gene expression discovery with spatial information.
  • To enable transcriptome analysis of specific wing areas during development.

Main Methods:

  • Laser capture microdissection of butterfly larval and pupal wing tissues.
  • Total RNA extraction without paraffin embedding or microtome use.
  • Transcriptome analysis of dissected tissue areas.

Main Results:

  • Successful RNA extraction from thick, fixed butterfly wing tissues.
  • Protocol bypasses common difficulties in RNA extraction for sequencing.
  • Provides spatial information alongside gene expression data.

Conclusions:

  • The developed laser dissection protocol is effective for spatial transcriptomics in butterfly wings.
  • This method facilitates the study of gene expression during development.
  • It offers a valuable tool for researchers investigating tissue-specific transcriptomes.