Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

6.7K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.7K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.8K
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

348
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
348

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Watchful waiting versus methylprednisolone or polidocanol injections as treatment of simple bone cysts.

Bone & joint open·2026
Same author

Deep Learning Predicts Mutations and Outcomes in Gastrointestinal Stromal Tumors from Whole-Slide Images.

Cancer research·2026
Same author

Critical appraisal of patient-reported and clinician-reported outcome measures assessing functional outcomes in lower extremity bone sarcoma patients-a COSMIN-based systematic review.

Journal of patient-reported outcomes·2026
Same author

Fibrosarcomatous Transformation of Dermatofibrosarcoma Protuberans: What Does It Mean and Why Does It Matter?

International journal of surgical pathology·2026
Same author

A Multicenter Retrospective Outcomes Analysis of Patients with Localized Synovial Sarcoma.

Cancer research communications·2026
Same author

Unplanned Excision and the Race to Cure Sarcomas: Commentary on an article by Jacob Jahn, BS, et al.: "Effect of Timing of First Consultation with a Sarcoma Specialist Following Unplanned Excision. Oncologic Outcomes of Patients with Soft-Tissue Sarcomas".

The Journal of bone and joint surgery. American volume·2026

Related Experiment Video

Updated: Aug 31, 2025

An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis
06:35

An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis

Published on: February 8, 2019

7.3K

Interactions in CSF1-Driven Tenosynovial Giant Cell Tumors.

David G P van IJzendoorn1, Magdalena Matusiak1, Gregory W Charville1

  • 1Department of Pathology, Stanford University School of Medicine, Stanford, California.

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
|August 25, 2022
PubMed
Summary

Tenosynovial giant cell tumor (TGCT) neoplastic cells resemble synoviocytes and lack CSF1R, suggesting current therapies may be ineffective. GFPT2 highlights these cells, pointing to new therapeutic targets like the YAP1/TAZ pathway.

More Related Videos

Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts
07:50

Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts

Published on: May 25, 2018

8.4K
Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs
12:44

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs

Published on: January 27, 2023

3.9K

Related Experiment Videos

Last Updated: Aug 31, 2025

An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis
06:35

An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis

Published on: February 8, 2019

7.3K
Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts
07:50

Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts

Published on: May 25, 2018

8.4K
Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs
12:44

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs

Published on: January 27, 2023

3.9K

Area of Science:

  • Oncology
  • Cell Biology
  • Genomics

Background:

  • Tenosynovial giant cell tumor (TGCT) involves neoplastic cells overproducing CSF1, stimulating bystander macrophages.
  • An autocrine loop via CSF1R on neoplastic cells was hypothesized but unconfirmed.

Purpose of the Study:

  • Investigate cellular interactions in TGCT using single-cell RNA sequencing (scRNA-seq).
  • Characterize neoplastic cell populations and their molecular profiles.
  • Identify potential therapeutic targets in TGCT.

Main Methods:

  • scRNA-seq on 18,788 cells from TGCT and GCTB samples.
  • Long-read RNA sequencing for TGCT samples.
  • Immunofluorescence and immunohistochemistry (IHC) for validation.

Main Results:

  • Identified two neoplastic cell populations in TGCT, similar to nonneoplastic synoviocytes.
  • Discovered GFPT2 as a marker for TGCT neoplastic cells.
  • Confirmed neoplastic cells lack CSF1R expression, challenging the autocrine loop hypothesis.

Conclusions:

  • TGCT neoplastic cells are similar to synoviocytes and may not respond to CSF1R-targeted therapies.
  • High GFPT2 expression correlates with YAP1/TAZ pathway activation.
  • Platelet-derived growth factor receptor expression suggests novel therapeutic avenues.