Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

178
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
178
CRISPR01:59

CRISPR

52.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.7K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intracellular acidification by microbiota-derived valeric acid facilitates trans-kingdom ecology limiting Candida parapsilosis colonization.

Cell host & microbe·2026
Same author

Genome sequence of the yeast <i>Candida sake</i> UCD2293, isolated from soil in Ireland.

Microbiology resource announcements·2026
Same author

Genome sequence of the yeast <i>Candida solani</i> UCD2211 isolated from soil in Ireland.

Microbiology resource announcements·2026
Same author

Emerging antifungal resistance in Candida parapsilosis: the end of the innocence.

npj antimicrobials and resistance·2025
Same author

Centromeres in budding yeasts are conserved in chromosomal location but not in structure.

PLoS genetics·2025
Same author

<i>Cyberlindnera hibernica</i> sp. nov. and <i>Barnettozyma discipulorum</i> sp. nov., isolated from forest soil in Ireland.

International journal of systematic and evolutionary microbiology·2025

Related Experiment Video

Updated: Aug 31, 2025

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
09:56

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

12.0K

Plasmid-Based CRISPR-Cas9 Editing in Multiple Candida Species.

Lisa Lombardi1, Geraldine Butler2

  • 1School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Dublin, Ireland. lisa.lombardi@ucd.ie.

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2022
PubMed
Summary

This study introduces efficient CRISPR-Cas9 gene editing tools for four non-albicans Candida species. These plasmid-based systems enable precise genetic manipulation for research into fungal pathogenesis and drug resistance.

Keywords:
CRISPR-Cas9Candida metapsilosisCandida orthopsilosisCandida parapsilosisCandida tropicalisGene deletionGene editingYeast transformation

More Related Videos

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

17.2K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.0K

Related Experiment Videos

Last Updated: Aug 31, 2025

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans
09:56

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

12.0K
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

17.2K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.0K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • CRISPR-Cas9 technology has revolutionized genetic manipulation in medically and industrially important fungi.
  • Applications span pathogenesis, drug resistance, gene expression, and host-pathogen interactions.

Purpose of the Study:

  • To describe efficient CRISPR-Cas9 gene editing systems for four non-albicans Candida species.
  • To enable genetic manipulation in Candida parapsilosis, Candida orthopsilosis, Candida metapsilosis, and Candida tropicalis.

Main Methods:

  • Utilized plasmid-based systems (pCP-tRNA and pCT-tRNA) for CRISPR-Cas9 expression.
  • Employed repair templates for gene disruption via premature stop codons or gene deletion.
  • Leveraged plasmid instability under non-selective conditions for scarless editing.

Main Results:

  • Achieved high-efficiency CRISPR-Cas9 gene editing in target Candida species.
  • Demonstrated successful gene disruption and deletion using the described plasmid systems.
  • Facilitated scarless gene editing by utilizing plasmids that are easily lost without selection.

Conclusions:

  • Developed versatile and efficient CRISPR-Cas9 tools for genetic studies in non-albicans Candida.
  • The described methods facilitate in-depth investigation of fungal biology and drug discovery.
  • Minimized detrimental effects of Cas9 expression through easily curable plasmids.