Consistent chromosome abnormalities in LS/BL murine lymphosarcoma cells

The LS/BL lymphosarcoma was induced by radiation in a C57BL/10 mouse in 1963 and was converted to ascites form in the first mouse transfer generation. In the course of cultivation in vivo the modal number of chromosomes dropped from the initial value 42-41 to 39 (73%). Cytogenetic characterization of the LS/BL tumor was carried out, using G-banding technique. Chromosome abnormalities were consistent in the cell line and involved chromosomes Nos. 3, 6, 12, 13, 16, and X. The most frequent abnormality was the presence of three markers and trisomy of chromosome No. 16.