[Acid phosphatase in seminal fluid--method of estimation and diagnostic significance]

A new method for the estimation of acid phosphatase in seminal fluid based on the use of p-nitro-phenylphosphate is described as simple in practicability and appropriate for routine in clinics. The frequency distribution of acid phosphatase activity is specified with 66 samples showing a normal spermiogram. The values spread logarithmically with an average of 400 U/ml and a standard deviation ranging from 200 U/ml to 880 U/ml. Correlations between acid phosphatase content and general, cytological and biochemical spermiogram-parameters are investigated. A plain negative correlation with the pH (r = 0,4615, n = 250) and a very strong positive correlation with citrate (r = +0,9029, n = 200) turned out. The significance of these correlations is discussed. Among the biochemical parameters in spermiogram both citrate and acid phosphatase--each representing prostatic function--are equivalent in diagnostical significance.