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Updated: Aug 31, 2025
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Serum microRNA miR-491-5p/miR-206 Is Correlated with Poor Outcomes/Spontaneous Hemorrhagic Transformation after
Xindi Song1, Junfeng Liu1, Yanan Wang1
1Center of Cerebrovascular Diseases, Department of Neurology, West China Hospital of Sichuan University, Chengdu 610041, China.
Background:
It is unclear whether miR-491-5p, miR-206, miR-21-5p or miR-3123 are associated with functional outcomes and hemorrhagic transformation (HT) after acute ischemic stroke (AIS). In this study, we aimed to investigate the correlation between these four microRNAs and functional outcomes, as well as spontaneous HT after AIS; Methods: We included 215 AIS patients and retrospectively assayed for miR-21-5p, miR-206, miR-3123 and miR-491-5p levels in serum. Poor functional outcome was defined as a modified Rankin Scale score ≥ 3. Spontaneous HT referred to hemorrhage detected in follow-up brain imaging but not on admission, without reperfusion therapies. Logistic regression, generalized additive model and 2-piecewise regression model were used to explore the independent, non-linear correlation between miRNA expression levels and outcomes; Results: We included 215 AIS patients. Higher miR-491-5p level independently reduced the risk of poor functional outcomes at 1 year (OR 0.90, 95% CI 0.82-0.98, corrected p value = 0.044). Higher miR-206 level significantly increased the risk of spontaneous HT (OR 1.64, 95% CI 1.17-2.30, corrected p value = 0.016). There was a nonlinear correlation found between miR-491-5p level and 1 year outcome with an inflection point of 2.180, while an approximately linear correlation was observed with an inflection point of 2.037 between miR-206 level and spontaneous HT; Conclusions: Higher serum miR-491-5p level independently reduced risk of 1-year poor functional outcome of AIS patients. Higher serum miR-206 level independently increased the risk of spontaneous HT in AIS patients. These two miRNAs may be as the potential biomarkers for improving prognosis after AIS.
Insights
Higher serum miR-491-5p levels are linked to better functional outcomes in acute ischemic stroke (AIS) patients. Conversely, elevated miR-206 levels increase the risk of spontaneous hemorrhagic transformation (HT) after AIS.
Area of Science:
- Biochemistry
- Genetics
- Neurology
Background:
- The association between specific microRNAs (miRNAs) and functional outcomes or hemorrhagic transformation (HT) following acute ischemic stroke (AIS) remains unclear.
- Investigating novel biomarkers for predicting prognosis after AIS is crucial.
Purpose of the Study:
- To examine the correlation between serum levels of miR-491-5p, miR-206, miR-21-5p, and miR-3123 and functional outcomes in AIS patients.
- To assess the relationship between these miRNAs and the occurrence of spontaneous HT after AIS.
Main Methods:
- A retrospective analysis of 215 AIS patients was conducted.
- Serum levels of four specific miRNAs were measured.
- Statistical models, including logistic regression and generalized additive models, were employed to analyze correlations.
Main Results:
- Higher serum miR-491-5p levels were independently associated with reduced risk of poor functional outcomes at one year (OR 0.90, p=0.044).
- Elevated serum miR-206 levels were independently linked to an increased risk of spontaneous HT (OR 1.64, p=0.016).
- Non-linear correlations were observed between miR-491-5p and functional outcomes, and miR-206 and spontaneous HT.
Conclusions:
- Serum miR-491-5p may serve as a potential biomarker for improved functional prognosis in AIS patients.
- Serum miR-206 could be a predictive biomarker for spontaneous HT risk in AIS.
- These miRNAs show potential for guiding therapeutic strategies and improving patient outcomes after AIS.
