Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Precision Medicine for Lipids: The New US Guidelines for Dyslipidemia.

Clinical chemistry·2026
Same author

Establishment and functional analysis of an inducible Apoc2-knockout mouse model to investigate the role of Apoc2 in normal hematopoiesis.

Lab animal·2026
Same author

Measurement of low-density lipoprotein cholesterol and other circulating lipids in Brazil: a systematic literature review.

Clinica chimica acta; international journal of clinical chemistry·2026
Same author

High-Density Lipoprotein-Specific Phospholipid Efflux (HDL-SPE) Assay in Mice.

Molecular nutrition & food research·2026
Same author

In Reply to Inter-Instrument Imprecision Complements Bias in Evaluating LDL-C Method Performance.

Clinical chemistry·2026
Same author

Corrigendum to "Identification of a novel apoB variant in a family exhibiting hypocholesterolemia: Mechanistic insights" [J Clin Lipidol (2026) S1933-2874(26)00045-0, ahead of print].

Journal of clinical lipidology·2026

Related Experiment Video

Updated: Aug 31, 2025

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

9.4K

A Simple Fluorescent Cholesterol Labeling Method to Cryoprotect and Detect Plasma Lipoprotein-X.

Edward B Neufeld1, Lita A Freeman1, Vinay Durbhakula1

  • 1Lipoprotein Metabolism Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1765, USA.

Biology
|August 26, 2022
PubMed
Summary

A new method uses fluorescent cholesterol to detect abnormal Lipoprotein-X (LpX) particles in patient plasma. This aids in diagnosing familial LCAT deficiency and biliary cholestasis.

Keywords:
BODIPY-cholesterolcholestasisfamilial LCAT deficiencylipoprotein-Xtrehalose

More Related Videos

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
07:33

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

6.5K
LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
08:45

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Published on: November 17, 2018

13.5K

Related Experiment Videos

Last Updated: Aug 31, 2025

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

Published on: October 12, 2017

9.4K
Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
07:33

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

6.5K
LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
08:45

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Published on: November 17, 2018

13.5K

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Lipidology

Background:

  • Lipoprotein-X (LpX) are abnormal, nephrotoxic lipoprotein particles characterized by high free cholesterol and phospholipid content.
  • Distinct LpX compositions arise in familial LCAT deficiency (FLD) and biliary cholestasis.
  • Standard lipid stains struggle to detect LpX due to its unique composition and instability.

Purpose of the Study:

  • To develop a simplified, sensitive method for detecting LpX in human plasma.
  • To enable detection in both fresh and cryopreserved samples.
  • To facilitate clinical diagnosis and treatment monitoring for FLD and cholestatic liver disease.

Main Methods:

  • Utilized fluorescent cholesterol complexed with fatty-acid-free BSA to label LpX.
  • Incorporated trehalose for cryopreservation of plasma LpX.
  • Employed agarose gel electrophoresis to separate and visualize fluorescently labeled LpX.

Main Results:

  • Successfully labeled LpX with fluorescent cholesterol, allowing sensitive detection.
  • Demonstrated the method's efficacy on both fresh and freeze-thawed plasma from FLD and cholestatic patients.
  • Observed high sensitivity in detecting fluorescent cholesterol bound to LpX post-electrophoresis.

Conclusions:

  • Developed a simplified, qualitative assay for LpX detection.
  • The method is suitable for fresh and cryopreserved samples, addressing LpX instability.
  • This assay can aid in diagnosing FLD, biliary liver disease, and monitoring therapeutic interventions.