Related Experiment Video
Updated: Aug 30, 2025
Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
Published on: December 5, 2017
Proposal for a New Diagnostic Histopathological Approach in the Evaluation of Ki-67 in GEP-NETs
Pinuccia Faviana1, Laura Boldrini1, Carlo Gentile2
1Department of Surgical, Medical, Molecular Pathology and Critical Area, University of Pisa, 56126 Pisa, Italy.
Introduction:
Studies have shown that the Ki-67 index is a valuable biomarker for the diagnosis, and classification of gastro-entero-pancreatic neuroendocrine tumors (GEP-NETs). We re-evaluated the expression of Ki-67 based on the intensity of the stain, basing our hypothesis on the fact that the Ki-67 protein is continuously degraded.
Background:
The aim was to evaluate whether a new scoring method would be more effective in classifying NETs by reducing staining heterogeneity.
Methods:
Patients with GEP-NET (n = 87) were analyzed. The classification difference between the two methods was determined.
Results:
The classification changed significantly when the Ki-67 semiquantal index was used. The percentage of G1 patients increased from 18.4% to 60.9%, while the G2 patients decreased from 66.7% to 29.9% and the G3 patients also decreased from 14.9% to 9.2%. Moreover, it was found that the traditional Ki-67 was not significantly related to the overall survival (OS), whereas the semiquantal Ki-67 was significantly related to the OS.
Conclusions:
The new quantification was a better predictor of OS and of tumor classification. Therefore, it could be used both as a marker of proliferation and as a tool to map tumor dynamics that can influence the diagnosis and guide the choice of therapy.
