Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Regional differences in brain glucose content in graded hypoglycemia.

W Paschen, B K Siesjö, M Ingvar

    Neurochemical Pathology
    |October 1, 1986
    PubMed
    Summary

    Hypoglycemia causes brain glucose levels to drop, with some brain regions like the caudoputamen depleting faster than others. These regional differences in brain glucose may explain why certain brain structures are more vulnerable to low glucose.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Acidosis promotes the permeability transition in energized mitochondria: implications for reperfusion injury.

    Journal of neurotrauma·2001
    Same author

    Does long-term glucose infusion reduce brain damage after transient cerebral ischemia?

    Brain research·2001
    Same author

    Protein aggregation after focal brain ischemia and reperfusion.

    Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2001
    Same author

    Hyperglycemia enhances DNA fragmentation after transient cerebral ischemia.

    Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2001
    Same author

    Cyclosporin A, but not FK506, prevents the downregulation of phosphorylated Akt after transient focal ischemia in the rat.

    Brain research·2001
    Same author

    Early release of cytochrome C and activation of caspase-3 in hyperglycemic rats subjected to transient forebrain ischemia.

    Brain research·2001

    Area of Science:

    • Neuroscience
    • Biochemistry

    Background:

    • Hypoglycemia, or low blood glucose, poses a significant risk to brain function.
    • Understanding regional brain glucose metabolism is crucial for comprehending neuronal vulnerability during metabolic stress.

    Purpose of the Study:

    • To investigate regional brain glucose concentrations during graded insulin-induced hypoglycemia in rats.
    • To correlate brain tissue glucose levels with plasma glucose concentrations across different brain structures.

    Main Methods:

    • Insulin-induced graded hypoglycemia in anesthetized, ventilated rats.
    • In situ brain freezing for tissue preservation.
    • Bioluminescent technique for quantifying regional glucose concentration.

    Main Results:

    Related Experiment Videos

    • Brain tissue glucose closely correlated with plasma glucose levels (tissue/plasma ratio ~0.3).
    • Significant regional variations in glucose content were observed.
    • The caudoputamen showed the lowest glucose levels, becoming depleted at plasma glucose below 3 mumol/mL.
    • Neocortex, hippocampus, and cerebellum also exhibited very low glucose levels at <4 mumol/mL plasma glucose.

    Conclusions:

    • Regional differences in brain glucose levels exist during hypoglycemia.
    • These inhomogeneities may contribute to the differential vulnerability of brain structures to ischemic damage.