Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ultrasound II: Endoscopic Ultrasound and FibroScan01:25

Ultrasound II: Endoscopic Ultrasound and FibroScan

188
Endoscopic Ultrasound (EUS) and FibroScan are valuable diagnostic tools in gastroenterology and hepatology, each with specific applications and techniques.
Endoscopic Ultrasound (EUS):
188

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early oligodendrocyte dysfunction signature in Alzheimer's disease: Insights from DNA methylomics and transcriptomics.

Molecular psychiatry·2026
Same author

No Improvement in Infection or Complication Rate With Extended Oral Antibiotic Prophylaxis After Primary Total Joint Arthroplasty.

The Journal of arthroplasty·2026
Same author

Posterior spinal fusion outcomes in adolescent idiopathic scoliosis patients with attention deficit hyperactivity disorder (ADHD): ADHD subtype makes a difference.

Spine deformity·2026
Same author

FKBP5 regulates interferon signaling leading to myeloid cell activation in multiple sclerosis.

Journal of neuroinflammation·2026
Same author

Autophagy Activators Normalize Aberrant Tau Proteostasis and Rescue Synapses in Human Familial Alzheimer's Disease iPSC-Derived Cortical Organoids.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Early-Life Nutritional Determinants of Pediatric MASLD.

Nutrients·2025

Related Experiment Video

Updated: Aug 30, 2025

Application of Ultrasound and Shear Wave Elastography Imaging in a Rat Model of NAFLD/NASH
07:13

Application of Ultrasound and Shear Wave Elastography Imaging in a Rat Model of NAFLD/NASH

Published on: April 20, 2021

4.1K

Hepatic PEMT Expression Decreases with Increasing NAFLD Severity.

Ignazio S Piras1, Anish Raju1, Janith Don1

  • 1Translational Genomics Research Institute, Phoenix, AZ 85004, USA.

International Journal of Molecular Sciences
|August 26, 2022
PubMed
Summary
This summary is machine-generated.

Reduced expression of phosphatidylethanolamine N-methyltransferase (PEMT) is linked to nonalcoholic fatty liver disease (NAFLD) severity. Lower PEMT levels correlate with inflammation, fibrosis, and cirrhosis in NAFLD patients, suggesting a role in disease progression.

Keywords:
cholinegene expressiongenetic variantshepatic steatosismenopausenonalcoholic steatohepatitisobesity

More Related Videos

Novel In Vivo Micro-Computed Tomography Imaging Techniques for Assessing the Progression of Non-Alcoholic Fatty Liver Disease
08:41

Novel In Vivo Micro-Computed Tomography Imaging Techniques for Assessing the Progression of Non-Alcoholic Fatty Liver Disease

Published on: March 24, 2023

1.3K
Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
09:15

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Published on: July 18, 2019

9.0K

Related Experiment Videos

Last Updated: Aug 30, 2025

Application of Ultrasound and Shear Wave Elastography Imaging in a Rat Model of NAFLD/NASH
07:13

Application of Ultrasound and Shear Wave Elastography Imaging in a Rat Model of NAFLD/NASH

Published on: April 20, 2021

4.1K
Novel In Vivo Micro-Computed Tomography Imaging Techniques for Assessing the Progression of Non-Alcoholic Fatty Liver Disease
08:41

Novel In Vivo Micro-Computed Tomography Imaging Techniques for Assessing the Progression of Non-Alcoholic Fatty Liver Disease

Published on: March 24, 2023

1.3K
Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
09:15

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Published on: July 18, 2019

9.0K

Area of Science:

  • Hepatology
  • Genetics
  • Metabolic Diseases

Background:

  • Choline deficiency induces hepatic fat accumulation, increasing nonalcoholic fatty liver disease (NAFLD) risk and fibrosis.
  • Reduced hepatic phosphatidylethanolamine N-methyltransferase (PEMT) expression is a known cause of steatosis, inflammation, and fibrosis in mouse models.
  • Common PEMT genetic variants in humans are associated with impaired phosphatidylcholine synthesis and elevated NAFLD risk.

Purpose of the Study:

  • To investigate hepatic PEMT expression across the spectrum of NAFLD in a large patient cohort.
  • To examine the relationship between PEMT genetic variants and their expression in the liver.
  • To determine if PEMT expression levels correlate with NAFLD severity, inflammation, and fibrosis.

Main Methods:

  • Analysis of hepatic PEMT expression in a large cohort of patients with varying degrees of NAFLD.
  • Correlation of PEMT expression levels with histological findings, including steatosis, inflammation, and fibrosis.
  • Investigation of associations between common PEMT genetic variants and hepatic PEMT gene expression.
  • Computational fine mapping to identify potential regulatory variants impacting PEMT expression.

Main Results:

  • Hepatic PEMT expression was significantly reduced in NAFLD patients with nonalcoholic steatohepatitis (NASH) compared to those with normal liver histology (p = 0.005).
  • PEMT levels demonstrated a negative correlation with increasing fibrosis severity, including bridging fibrosis, incomplete cirrhosis, and cirrhosis (p = 0.011).
  • Reduced hepatic PEMT expression was observed in postmenopausal women with NASH compared to controls (p = 0.030).
  • A suggestive association was found between the rs7946 variant and hepatic fibrosis (p = 0.083), and rs4646385 was computationally predicted to impact liver PEMT levels.

Conclusions:

  • Hepatic PEMT expression decreases with increasing severity of NAFLD, particularly in obese individuals and postmenopausal women.
  • Reduced PEMT expression may play a significant role in the pathogenesis of NASH and liver fibrosis in a subset of patients.
  • Further research into PEMT's role could identify novel therapeutic targets for NAFLD and related liver conditions.