Transcriptome Analysis and Intraspecific Variation in Spanish Fir (Abies pinsapo Boiss.)

Area of Science:

• Molecular biology
• Plant genomics
• Conservation genetics

Background:

• Spanish fir (Abies pinsapo) is an endangered, endemic conifer with limited molecular investigation.
• Two distinct needle phenotypes (green and bluish-green) exist within its largest population.
• Understanding genetic and molecular variations is crucial for conservation.

Purpose of the Study:

• To assemble the Spanish fir transcriptome for functional genomics.
• To investigate the molecular and physiological basis of green vs. bluish needle phenotypes.
• To provide resources for conservation and adaptive management strategies.

Main Methods:

• Transcriptome assembly and gene cataloging.
• Physiological marker analysis (electron transport, pigment content, nitrogen, cellulose).
• Differential gene expression analysis and epigenomic profile investigation.

Main Results:

• A comprehensive transcriptome resource with 22,769 expressed genes was generated.
• Green needles showed higher chlorophyll, protein, nitrogen, and electron transport efficiency.
• Bluish needles had higher carotenoid and cellulose content, suggesting nitrogen imbalance and potential epigenomic differences.

Conclusions:

• The study provides valuable transcriptome data for Spanish fir functional genomics and genome annotation.
• Identified physiological and transcriptomic differences between needle phenotypes offer insights into natural variation.
• Findings support improved conservation guidelines and adaptive management for Spanish fir under climate change.