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Related Experiment Video

Updated: Aug 30, 2025

An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach
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Subretinal Injection Techniques for Retinal Disease: A Review.

Cristina Irigoyen1,2,3, Asier Amenabar Alonso1, Jorge Sanchez-Molina1

  • 1Department of Ophthalmology, Donostia University Hospital (HUD), 20014 Donostia San-Sebastián, Spain.

Journal of Clinical Medicine
|August 26, 2022
PubMed
Summary

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Gene therapy offers hope for inherited retinal dystrophies (IRDs). Subretinal injection is a key delivery method, and standardizing this technique is crucial for successful treatment of IRDs.

Area of Science:

  • Ophthalmology
  • Genetics
  • Regenerative Medicine

Background:

  • Inherited retinal dystrophies (IRDs) affect approximately 1 in 2000 people globally, with 270 associated genes identified.
  • Most IRDs impact photoreceptor and retinal pigment epithelium function, leading to vision loss.
  • Gene therapy presents a promising therapeutic strategy for IRDs, with successful clinical trials and FDA-approved treatments like voretigene neparvovec.

Purpose of the Study:

  • To review current subretinal injection techniques used in gene therapy for IRDs.
  • To identify critical factors influencing the efficacy and safety of subretinal delivery.
  • To highlight the need for procedural standardization in the evolving field of retinal gene therapy.

Main Methods:

  • Review of existing scientific literature on subretinal injection methods for gene therapy.
Keywords:
retinal gene therapysubretinal injectionsubretinal injection techniquevitreoretinal surgery

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Last Updated: Aug 30, 2025

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  • Analysis of techniques for delivering viral vectors to photoreceptors and retinal pigment epithelium.
  • Discussion of factors contributing to successful subretinal delivery.

    • Main Results:

    • Multiple effective subretinal injection approaches exist for accessing the target space.
    • Subretinal injection is increasingly utilized for delivering gene therapy vectors.
    • Standardization of subretinal injection is needed to ensure consistent outcomes.

    Conclusions:

    • Subretinal injection is a vital technique for delivering gene therapy to treat IRDs.
    • Standardizing subretinal injection procedures is essential for maximizing treatment efficacy and patient safety.
    • Further research into optimizing subretinal delivery methods will advance gene therapy for retinal diseases.