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Pressure-Driven Perfusion System to Control, Multiplex and Recirculate Cell Culture Medium for Organs-on-Chips
Mees N S de Graaf1, Aisen Vivas2,3, Andries D van der Meer2
1Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands.
Micromachines
|August 26, 2022
Summary
We developed a novel method for multiplexing microfluidic channels in organ-on-chip devices, enabling high-throughput drug testing. This system also supports medium recirculation, reducing costs for expensive reagents.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Microfluidics
Background:
- Organ-on-chip (OoC) devices simulate organ microenvironments for research.
- Current microfluidic flow control methods (gravity, rockers, pumps) limit high-throughput applications.
- Consistent flow is crucial for accurate cell culture and drug response testing.
Purpose of the Study:
- To present a new method for multiplexing microfluidic channels in OoC devices.
- To introduce custom software for operating the multiplexed system.
- To demonstrate the system's suitability for medium recirculation.
Main Methods:
- Development of a novel multiplexing technique for microfluidic channels.
- Creation of custom software for system control.
- Validation of medium recirculation for OoC systems.
Main Results:
- The new method enables multiplexing of microfluidic channels in OoC devices.
- Custom software facilitates system operation.
- The approach supports efficient medium recirculation, reducing reagent costs.
Conclusions:
- This method enhances the throughput of organ-on-chip systems for drug screening.
- Medium recirculation capability offers significant cost-saving benefits.
- The developed system advances the utility of OoC technology in pharmaceutical research.
