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Isorhamnetin Suppresses Human Gastric Cancer Cell Proliferation through Mitochondria-Dependent Apoptosis

Yehua Li1, Baoqiang Fan1, Ning Pu1

  • 1College of Life Science, Northwest Normal University, Lanzhou 730070, China.

Molecules (Basel, Switzerland)
|August 26, 2022
PubMed

Insights

Isorhamnetin (ISO) effectively inhibits gastric cancer (GC) cell proliferation by inducing apoptosis through the mitochondria-dependent pathway. This natural compound shows promise as a potential therapeutic strategy for gastric cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Natural product derivatives are crucial in cancer therapy.
  • Isorhamnetin (ISO), a flavonoid, exhibits potential anti-tumor properties.
  • The efficacy of ISO in gastric cancer (GC) requires further investigation.

Purpose of the Study:

  • To investigate the anti-cancer effects of Isorhamnetin (ISO) on gastric cancer (GC) cells.
  • To elucidate the underlying molecular mechanisms of ISO's action in GC.
  • To assess the therapeutic potential of ISO for GC treatment.

Main Methods:

  • In vitro and in vivo proliferation assays using GC cell lines (AGS-1, HGC-27).
  • Transcriptomic analysis to identify differentially expressed genes.
  • Western blotting and assays to evaluate apoptosis-related proteins (caspase-3, Bax, Bcl-2, PARP), mitochondrial potential, and reactive oxygen species (ROS).

Main Results:

  • ISO significantly inhibited GC cell proliferation in a time- and dose-dependent manner.
  • Transcriptomic analysis revealed enrichment of genes related to cell growth and apoptosis.
  • ISO activated the caspase-3 cascade, altered Bax/Bcl-2 expression, increased cytosolic cytochrome c, and reduced mitochondrial potential, leading to apoptosis.

Conclusions:

  • ISO induces apoptosis in gastric cancer cells via the mitochondria-dependent pathway.
  • The caspase-3 cascade plays a critical role in ISO-mediated GC cell death.
  • ISO represents a potential therapeutic agent for clinical gastric cancer treatment.
Keywords:
apoptosisgastric cancerisorhamnetinmitochondriatranscriptome

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