Monoterpenoids Evolution and MEP Pathway Gene Expression Profiles in Seven Table Grape Varieties

Area of Science:

• Plant Biochemistry
• Molecular Biology
• Agricultural Science

Background:

• Monoterpenoids contribute significantly to the aroma and flavor profiles of table grapes.
• Understanding the biosynthesis and accumulation of these compounds is crucial for grape breeding and quality control.

Purpose of the Study:

• To investigate the evolution of monoterpenoids and their biosynthesis gene expression in the MEP pathway during table grape development.
• To evaluate the correlation between monoterpenoid accumulation and gene expression in different grape varieties.

Main Methods:

• Analysis of monoterpenoid profiles in seven table grape varieties over two seasons from veraison to maturity.
• Quantitative analysis of gene expression related to the MEP pathway.
• Pearson's correlation analysis to determine relationships between gene expression and monoterpenoid levels.

Main Results:

• Linalool, geraniol, and linalool oxides were identified as major monoterpenoids in Muscat varieties.
• 'Zaomeiguixiang' and 'Xiangfei' exhibited distinct monoterpenoid accumulation patterns.
• The MEP pathway gene VvDXS3 showed a significant correlation with monoterpenoid accumulation.

Conclusions:

• Monoterpenoid volatiles exhibit distinct evolutionary patterns during table grape berry development.
• VvDXS3 is a key candidate gene influencing monoterpenoid synthesis in table grapes.