CircRNA circ-PDCD11 is highly expressed in lung large-cell carcinoma and predicts poor survival
Tinghang Ma1, Hui Miao2, Yongzhong Xiong3
1Cancer Center, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan City, Shandong Province, P. R. China.
Background:
Circ-PDCD11 (hsa_circ_0019853, 461 bp) has been characterized as an oncogenic circRNA in breast cancer, while its function in other cancers is unclear. In this study, we explored the role of circ-PDCD11 in lung cancer.
Methods:
Plasma samples were obtained from patients with lung large-cell carcinoma (LLCC, n = 40), lung squamous cell carcinoma (LSCC, n = 40), lung adenocarcinoma (LA, n = 40) and small-cell lung cancer (SCLC, n = 40) as well as healthy controls (Control, n = 40). Paired tumor and nontumor tissue samples were obtained from all patients. Expression of circ-PDCD11 in these samples was determined by RT-qPCR. The role of plasma circ-PDCD11 in the diagnosis of LLCC was analyzed with ROC curve. A five-year follow-up was performed to analyze the role of plasma circ-PDCD11 in the prognosis of LLCC.
Results:
Plasma circ-PDCD11 was specifically upregulated in LLCC but not in other lung cancer types, compared to the controls. Increased circ-PDCD11 expression in tumor tissues compared to nontumor tissues was only observed in LLCC patients but not in other lung cancer types. Increased plasma circ-PDCD11 levels effectively separated LLCC patients from patients with other types of cancers. High plasma circ-PDCD11 levels were closely correlated with poor survival of LLCC patients. Plasma circ-PDCD11 levels were closely correlated with tumor metastasis, but not tumor size of LLCC.
Conclusion:
CircRNA circ-PDCD11 is highly expressed specifically in LLCC and predicts poor survival.
