Related Experiment Video

Updated: Aug 18, 2026

RhoC GTPase Activation Assay
09:58

RhoC GTPase Activation Assay

Published on: August 22, 2010

Activated Ki-ras oncogene in human prostatic adenocarcinoma

D M Peehl, N Wehner, T A Stamey

    The Prostate
    |January 1, 1987
    PubMed

    Insights

    Investigating cellular oncogenes in prostate cancer revealed a rare activation of Ki-ras in one sample. Most prostate cancers did not show detectable oncogene activation or amplification, suggesting limited involvement of these specific oncogenes.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Genetics

    Background:

    • The role of cellular oncogenes in human prostate cancer development requires further investigation.
    • Understanding oncogene activation is crucial for identifying potential therapeutic targets in prostate adenocarcinoma.

    Purpose of the Study:

    • To identify activated oncogenes in human prostate cancer tissues.
    • To determine the frequency of oncogene activation and amplification in prostatic adenocarcinoma.

    Main Methods:

    • DNA from prostatic adenocarcinoma tissues was tested for transforming activity using a 3T3 transfection assay.
    • Genomic DNA was analyzed for amplification of specific oncogenes, including Ki-ras, Ha-ras, c-myc, N-myc, c-sis, and c-fos.

    Main Results:

    • A transforming sequence homologous to Ki-ras was detected in one prostate cancer sample.
    • The majority of tested prostate cancer DNA samples were negative in the 3T3 transformation assay.
    • No amplification of the examined genomic oncogene sequences was detected in any of the prostatic tissue samples.

    Conclusions:

    • Activation of cellular oncogenes, detectable by the 3T3 transfection assay, appears infrequent in human prostate cancer.
    • Amplification of common oncogenes (Ki-ras, Ha-ras, c-myc, N-myc, c-sis, c-fos) is not a detectable event in the studied prostate cancer tissues.

    More Related Videos

    Pre-clinical Orthotopic Murine Model of Human Prostate Cancer07:01

    Pre-clinical Orthotopic Murine Model of Human Prostate Cancer

    Published on: August 29, 2016

    In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System05:45

    In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System

    Published on: January 9, 2020

    Related Concept Videos

    Abnormal Proliferation02:23

    Abnormal Proliferation

    Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
    The Ras Gene02:38

    The Ras Gene

    The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
    Ras is a superfamily...
    mTOR Signaling and Cancer Progression03:03

    mTOR Signaling and Cancer Progression

    The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
    The mTOR pathway or the...
    Rous Sarcoma Virus (RSV) and Cancer01:03

    Rous Sarcoma Virus (RSV) and Cancer

    Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
    RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
    The Retinoblastoma Gene01:20

    The Retinoblastoma Gene

    Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
    The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
    Rous Sarcoma Virus (RSV) and Cancer01:03

    Rous Sarcoma Virus (RSV) and Cancer

    Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
    RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...