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Activated Ki-ras oncogene in human prostatic adenocarcinoma
The Prostate
|January 1, 1987
Summary
Investigating cellular oncogenes in prostate cancer revealed a rare activation of Ki-ras in one sample. Most prostate cancers did not show detectable oncogene activation or amplification, suggesting limited involvement of these specific oncogenes.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The role of cellular oncogenes in human prostate cancer development requires further investigation.
- Understanding oncogene activation is crucial for identifying potential therapeutic targets in prostate adenocarcinoma.
Purpose of the Study:
- To identify activated oncogenes in human prostate cancer tissues.
- To determine the frequency of oncogene activation and amplification in prostatic adenocarcinoma.
Main Methods:
- DNA from prostatic adenocarcinoma tissues was tested for transforming activity using a 3T3 transfection assay.
- Genomic DNA was analyzed for amplification of specific oncogenes, including Ki-ras, Ha-ras, c-myc, N-myc, c-sis, and c-fos.
Main Results:
- A transforming sequence homologous to Ki-ras was detected in one prostate cancer sample.
- The majority of tested prostate cancer DNA samples were negative in the 3T3 transformation assay.
- No amplification of the examined genomic oncogene sequences was detected in any of the prostatic tissue samples.
Conclusions:
- Activation of cellular oncogenes, detectable by the 3T3 transfection assay, appears infrequent in human prostate cancer.
- Amplification of common oncogenes (Ki-ras, Ha-ras, c-myc, N-myc, c-sis, c-fos) is not a detectable event in the studied prostate cancer tissues.