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Updated: Aug 30, 2025

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
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A rapid method for extracting microplastics from oily food samples.

Cui-Lan Bai1, Ting-Ting Xu1, Ying Guo1

  • 1Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China. yingguo2004@jnu.edu.cn.

Analytical Methods : Advancing Methods and Applications
|August 26, 2022
PubMed
Summary
This summary is machine-generated.

A new method rapidly extracts microplastics (MPs) from oily foods using nitric acid and hydrogen peroxide. This efficient technique achieves high recovery rates, aiding in the analysis of plastic contamination in food.

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Food Science

Background:

  • Microplastic (MP) contamination impacts aquatic life, yet methods for extracting MPs from oily foods are scarce.
  • Existing digestion methods for oily foods are time-consuming and risk procedural contamination.

Purpose of the Study:

  • To develop a rapid, efficient, economical, and simple analytical method for extracting MPs from oily food samples.
  • To address the need for standardized methods in MP analysis within the food industry.

Main Methods:

  • A novel protocol utilizing a 4:1 HNO3:H2O2 digestion reagent at 50 °C for 1 hour.
  • Hexane was employed as a washing solution to eliminate oil adsorbed onto MPs and membranes.
  • The method was validated using common MPs (PET, PE, PS, PP) spiked into oily flours.

Main Results:

  • High mean MP recovery of 95% ± 2% (93-98%) was achieved.
  • Recovered polymers showed no significant changes in physical or spectral properties, except minor alterations in PS.
  • The method demonstrated effectiveness on diverse food matrices including rice, noodles, bean products, and meats.

Conclusions:

  • The developed method facilitates efficient observation and identification of MP characteristics in oily foods.
  • This innovative approach supports both quantitative and qualitative analyses of microplastic contamination in food samples.
  • The protocol offers a significant advancement for monitoring plastic pollution in the food supply chain.