Related Experiment Video
Updated: Aug 30, 2025
10:09
Large-scale Scanning Transmission Electron Microscopy Nanotomy of Healthy and Injured Zebrafish Brain
Published on: May 25, 2016
11.2K
Neurogranin-like immunoreactivity in the zebrafish brain during development
Anabel Alba-González1,2, Julián Yáñez3,4, Ramón Anadón5
1Department of Biology, Faculty of Sciences, University of A Coruña, Campus da Zapateira, 15008-A, Coruña, Spain.
Brain Structure & Function
|August 26, 2022
Summary
Neurogranin (Nrgn) expression develops late in zebrafish neurons, appearing by 2 days post-fertilization and reaching adult patterns by 6 days. This protein marks specific neuronal circuits in the developing brain and sensory structures.
Area of Science:
- Neuroscience
- Developmental Biology
- Molecular Biology
Background:
- Neurogranin (Nrgn) is a neural protein crucial for synaptic plasticity, interacting with calmodulin.
- Previous research established Nrgn expression in the adult zebrafish brain.
Purpose of the Study:
- To investigate the developmental expression pattern of Nrgn-like immunoreactivity (Nrgn-like-ir) in zebrafish embryos and larvae.
- To identify specific brain regions and sensory structures where Nrgn expression emerges during development.
Main Methods:
- Utilized whole-mount and section immunohistochemistry to detect Nrgn-like-ir in zebrafish.
- Analyzed the temporal and spatial distribution of Nrgn-like-ir from 2 days post-fertilization (dpf) onwards.
Main Results:
- Nrgn-like-ir was first detected at 2 dpf in the pallium, epiphysis, and hindbrain.
- Expression expanded significantly by 3 dpf and reached adult-like patterns by 6 dpf.
- Nrgn-like-ir was prominent in olfactory organs, retina, pallium, hypothalamus, thalamus, optic tectum, and other sensory/visceral areas, including axonal tracts.
Conclusions:
- Late Nrgn expression in zebrafish neurons correlates with the functional maturation of higher brain centers.
- Nrgn serves as a potential marker for delineating specific neuronal circuitries in the developing zebrafish brain.
