Ultrathin trimetallic metal-organic framework nanosheets for accelerating bacteria-infected wound healing

Bacteria-infected wounds are commonly regarded as a hidden threat to human health that can create persistent infection and even bring about amputation or death. Two-dimensional metal-organic frameworks (2D MOFs) with biomimetic enzyme activity have been used to reduce the huge harm caused by antibiotic resistance due to their massive active sites and ultralarge specific surface area. However, their therapeutic efficiency is unsatisfactory because of their relatively low catalytic activity and poor productivity. In this paper, we presented a simple and mild one-pot solution phase method for the large-scale synthesis of NiCoCu-based MOF nanosheets. The NiCoCu nanosheets (denoted as (Ni₂Co₁)_1-xCu_x) with controlled molar ratios have different morphologies and sizes. Specifically, the (Ni₂Co₁)_0.5Cu_0.5 nanosheets showed the best catalytic performance toward the reduction of H₂O₂ and H₂O₂ was efficiently catalyzed to generate toxic •OH in the presence of MOF nanosheets with peroxidase-like activity. (Ni₂Co₁)_0.5Cu_0.5 exhibited the best antibacterial activity against gram-positive Escherichia coli and methicillin-resistant Staphylococcus aureus bacteria. Animal wound healing experiments demonstrate that ultrathin trimetallic nanosheets can effectively contribute to wound healing with excellent biocompatibility. This study reveals the immense potential of ultrathin trimetallic MOF nanosheets for clinical antibacterial therapy for future pragmatic clinical applications.