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Updated: Aug 30, 2025
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Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
Published on: October 4, 2024
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Optogenetic manipulation of cell migration with high spatiotemporal resolution using lattice lightsheet microscopy
Wei-Chun Tang1, Yen-Ting Liu1, Cheng-Han Yeh1
1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
Communications Biology
|August 26, 2022
Summary
Optogenetic activation microscopy (optoLLSM) uses Bessel beams for precise cellular control. This technique enables subcellular manipulation and guided cell migration with low light power and minimal damage.
Area of Science:
- Biophysics
- Cell Biology
- Microscopy
Background:
- Lattice lightsheet microscopy (LLSM) enables 3D imaging.
- Optogenetic tools allow precise control of cellular functions.
- Subcellular resolution manipulation of cellular behavior is challenging.
Purpose of the Study:
- To enhance LLSM for subcellular optogenetic activation (optoLLSM).
- To achieve spatiotemporal control of cellular behavior with high resolution.
- To demonstrate guided cell migration using optogenetics.
Main Methods:
- Integration of a position-controllable Bessel beam into LLSM.
- Utilizing spatial light modulator (SLM) patterns for spatiotemporal photoactivation.
- Employing CRY2olig clustering proteins and phosphoinositide 3-kinase recruitment.
Main Results:
- Optogenetic activation achieved with low light power (< 1 nW).
- Subcellular induction of membrane ruffling and protein clustering demonstrated.
- Guided cell migration observed for up to 6 hours with minimal cellular damage.
Conclusions:
- OptoLLSM provides a powerful tool for subcellular manipulation of cellular behavior.
- The technique allows for precise control of cellular processes with high spatiotemporal resolution.
- Demonstrated guided cell migration highlights the potential for studying dynamic cellular processes.
