Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

9.1K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.1K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

178
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
178
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K
CRISPR01:59

CRISPR

52.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.7K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

6.5K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metagenomic insights into the mechanisms of heteroatom-doped, iron-loaded biochar in enhancing anaerobic digestion of waste activated sludge.

Bioresource technology·2026
Same author

Frataxin attenuates endothelial inflammation triggered by engulfment of senescent erythrocytes.

Free radical biology & medicine·2026
Same author

Xin-Zi-Sheng-Wan decoction alleviates hyperuricemia-associated renal injury by inhibiting RIPK1/RIPK3/MLKL-mediated necroptosis.

Journal of ethnopharmacology·2026
Same author

Mineral-biofilm interaction controls trophic transfer of PFAS along a biofilm-snail food chain.

Aquatic toxicology (Amsterdam, Netherlands)·2026
Same author

The patterns of microbial community distribution and co-occurrence in water columns and sediments of Haima cold seep.

Microbiology spectrum·2026
Same author

LIFT+: Lightweight Fine-Tuning for Long-Tail Learning.

IEEE transactions on pattern analysis and machine intelligence·2026

Related Experiment Video

Updated: Aug 30, 2025

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.2K

Programmable RNA base editing with a single gRNA-free enzyme.

Wenjian Han1,2, Wendi Huang1,2, Tong Wei1,2

  • 1Bio-Med Big Data Center, CAS Key Laboratory of Computational Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

Nucleic Acids Research
|August 27, 2022
PubMed
Summary
This summary is machine-generated.

A new gRNA-free RNA editing system called REWIRE uses a single engineered protein for precise gene expression control. This platform achieves high editing rates in human cells and mice, offering a versatile tool for genetic research and therapy.

More Related Videos

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.3K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.0K

Related Experiment Videos

Last Updated: Aug 30, 2025

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
07:31

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

1.2K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.3K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.0K

Area of Science:

  • Molecular Biology
  • Genetic Engineering
  • Biotechnology

Background:

  • Programmable RNA editing offers a way to modify gene expression without altering genomic DNA.
  • Existing RNA editing tools often rely on guide RNA (gRNA) assembly, leading to delivery challenges and reduced efficiency.

Purpose of the Study:

  • To develop a novel, gRNA-free RNA editing system for precise base editing.
  • To enhance RNA editing efficiency and specificity using engineered proteins.

Main Methods:

  • Development of REWIRE (RNA editing with individual RNA-binding enzyme), a single engineered protein system.
  • Incorporation of a human-derived PUF domain for specific RNA recognition and deaminase domains for A-to-I or C-to-U editing.
  • Optimization of the RNA-binding domain to improve editing outcomes and reduce off-target effects.

Main Results:

  • REWIRE achieved 60-80% editing rates in human cells.
  • Demonstrated precise A-to-I and C-to-U base editing with minimal off-target effects.
  • Successfully applied REWIRE to correct disease-associated mutations in mice.

Conclusions:

  • REWIRE is a precise and efficient single-component RNA editing platform derived from human proteins.
  • The system shows broad applicability for RNA base editing in cellular and in vivo models.
  • REWIRE overcomes delivery barriers associated with gRNA-based systems.