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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
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Nucleus-targeting pH-Responsive carbon dots for fast nucleus pH detection.

Samran Durrani1, Zihuayuan Yang1, Jie Zhang1

  • 1State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

Talanta
|August 27, 2022
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Summary

Researchers developed novel green emissive carbon dots (CDs) for real-time nucleus pH monitoring in living cells. This breakthrough enables long-term, wash-free imaging and sensing, crucial for understanding diseases linked to nuclear pH.", Enhanced_Abstract=default_api.SeocontentEnhancedAbstract(Area_of_Science=[

Keywords:
BiosensorCarbon quantum dotsIn-vivo pH sensorLong-rang pH sensorNucleus pH detectionNucleus tracking

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Nuclear pH is implicated in various diseases, including aging, cancer, and neurodegenerative disorders.
  • Direct monitoring of nucleus pH in living cells remains a significant challenge.
  • Existing fluorescent sensors lack nucleus-targeting capabilities and efficient detection methods.

Purpose of the Study:

  • To develop a novel fluorescent sensor for nucleus pH detection in living cells.
  • To investigate the nucleus-targeting and accumulation properties of the sensor.
  • To evaluate the sensor's performance for real-time and in vivo pH monitoring.

Main Methods:

  • Synthesis of green emissive carbon dots (CDs).
  • Cellular uptake studies using lipid raft mediated endocytosis for nucleus targeting.
  • Evaluation of pH-responsive fluorescence properties.
  • Live-cell imaging and in vivo sensing in zebrafish.

Main Results:

  • CDs selectively target the nucleus and accumulate in the nucleolus.
  • Achieved fast, wash-free, and long-term nucleus/nucleolus imaging.
  • Demonstrated pH-responsive fluorescence with linear ranges from pH 2-7 and pH 7-12.
  • Successfully applied CDs for nucleus pH detection in A549 cells and in vivo zebrafish.

Conclusions:

  • CDs are a promising fluorescent sensor for nucleus pH detection.
  • The sensor enables effective nucleus imaging and pH sensing in living systems.
  • This technology can advance the understanding of nucleus-related biological events and diseases.