Alkaloids, sesquiterpenoids and hybrids of terpenoid with p-hydroxycinnamic acid from Ganoderma sinensis and their...

The fruiting bodies of Ganoderma sinensis are used as food raw materials of marketed healthcare products. To gain an insight into the chemical and biological profling of G. sinensis, twenty-five compounds including eleven undescribed ones (ganodermasines A‒K) and fourteen known ones were isolated. Among them, ganodermasines A‒D are undescribed diketopiperazine alkaloids, ganodermasines E and F are alkaloids containing a pyridine. The structures of undescribed compounds were identified by spectroscopic, computational, and crystallographic methods. The results of acetylcholinesterase (AchE) inhibitory activity show that ganodermasines I and J could inhibit AchE with IC₅₀ values of 26.05 and 20.40 μM, respectively. In addition, neurotrophic assay in PC-12 cells showed that (+)-ganodermasine E, (-)-ganodermasine E, and ganodermasine I could stimulate neurite outgrowth at 10 μM, while the other isolates are inactive. The present findings will lend a hand for further utilization of G. sinensis.