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Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
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Limitations to Understanding Intestinal Stem Cell Activity via Cre-Lox-Based Lineage Tracing

Nicolette M Johnson1, Jeeyoon Na2, Keara E Monaghan2

  • 1Medical Scientist Training Program, Perelman School of Medicine, Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.

Cellular and Molecular Gastroenterology and Hepatology
|August 28, 2022
PubMed
Abstract

No abstract available in PubMed .

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The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
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