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Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry
Sebastian J Reich1, Jonas Stohr1, Oliver Goldbeck1
1Institute of Microbiology and Biotechnology, University of Ulm, Ulm, Germany.
Microbiologyopen
|August 29, 2022
Abstract:
The global increase in antibiotic resistance of pathogenic microorganisms requires the identification and characterization of novel antimicrobials. Bacterial biosensors expressing fluorescent proteins such as pHluorin variants are suitable for high-throughput screenings. Here, we present Listeria spp. pH-sensitive biosensors with improved fluorescence for single-cell analysis of antimicrobials by flow cytometry.

