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Related Concept Videos

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
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Lymph nodes are bean-shaped structures that cluster along the lymphatic vessels in the inguinal, axillary, and cervical regions. Each node is divided into compartments by a capsule that extends trabeculae inward.
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The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
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Related Experiment Video

Updated: Aug 30, 2025

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
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Characterization of CD41+ cells in the lymph node.

Li Dai1,2, Mayuko Uehara1, Xiaofei Li1

  • 1Transplantation Research Center, Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, United States.

Frontiers in Immunology
|August 29, 2022
PubMed
Summary

Researchers discovered novel CD41+ cells in lymph nodes (LNs) that are not platelets but express mesodermal and stromal markers. These cells recirculate and their trafficking is regulated by the S1P1 pathway, suggesting a potential role in LN immunity.

Keywords:
CD41 + progenitor cellslymph nodeslymphaticlymphatic remodelingstromal cells

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Isolation of Murine Lymph Node Stromal Cells
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Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification
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Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification

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Area of Science:

  • Immunology
  • Cell Biology
  • Hematology

Background:

  • Lymph nodes (LNs) are vital immune sites, with stromal cells playing a key role.
  • Recent research has identified novel stromal cell populations within LNs.
  • CD41, typically associated with platelets, is also found on other cell types.

Purpose of the Study:

  • To characterize novel CD41-expressing cells found in mouse and human lymph nodes.
  • To determine the origin, markers, and potential function of these CD41+ cells.
  • To investigate the trafficking and regulation of these newly identified LN cells.

Main Methods:

  • Flow cytometry and immunofluorescence to identify and characterize CD41+ cell populations.
  • Analysis of mesodermal and platelet markers.
  • Examination of thoracic duct lymph fluid.
  • Treatment with FTY720 to assess cell trafficking.
  • Radiation sensitivity assays.
  • Single-cell RNA sequencing.

Main Results:

  • Two distinct CD41+ cell populations (CD41+Lyve1+ and CD41+Lyve1-) were identified in LNs.
  • CD41+ cells expressed mesodermal markers (Sca-1, CD105, CD29) but lacked platelet markers.
  • CD41+Lyve1- cells were found in lymph fluid, indicating systemic recirculation.
  • FTY720 treatment reduced CD41+Lyve1- cell trafficking, implicating the S1P1 pathway.
  • CD41+Lyve1- cells showed sensitivity to radiation, suggesting replicative capacity.
  • Single-cell RNA sequencing indicated these cells express stromal cell markers.

Conclusions:

  • Novel CD41+ cells, distinct from platelets, reside in lymph nodes and recirculate.
  • These cells possess mesodermal and stromal characteristics, suggesting a role in LN stromal compartment.
  • Their trafficking is modulated by the S1P1 pathway.
  • Further research is needed to elucidate the precise function of CD41+ cells in LN immunity.