Related Experiment Video
Updated: Aug 30, 2025
12:19
Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin shRNA
Published on: February 12, 2020
8.0K
Buffalo long non-coding RNA gene11007 promotes myoblasts proliferation.
Ning Zhang1,2, Gaoxiao Xu3, Ping Sun4
1Ningxia Key Laboratory of Ruminant Molecular and Cellular Breeding, School of Agriculture, Ningxia University, Yinchuan, China.
Frontiers in Veterinary Science
|August 29, 2022
Summary
This study identifies long non-coding RNAs (lncRNAs) involved in buffalo skeletal muscle development. A specific lncRNA, gene11007, was found to promote myoblast proliferation, offering insights into buffalo meat quality regulation.
Area of Science:
- Animal Science
- Molecular Biology
- Genomics
Background:
- Buffalo meat quality is linked to skeletal muscle development, a process regulated by long non-coding RNAs (lncRNAs).
- The specific roles of lncRNAs in buffalo skeletal muscle growth and differentiation remain largely unexplored.
Purpose of the Study:
- To investigate the lncRNA expression profiles in buffalo myoblasts during proliferation and differentiation.
- To identify differentially expressed lncRNAs (DELs) and characterize their function in buffalo skeletal muscle development.
Main Methods:
- Ribo-Zero RNA-Sequencing was employed to profile lncRNAs in buffalo myoblasts.
- Quantitative PCR (qPCR) was used to validate DELs across different buffalo tissues and developmental stages.
- Functional assays including EdU, CCK-8, and Western blotting were performed to characterize gene11007.
Main Results:
- A total of 9,978 lncRNAs were identified, with 1,576 DELs found during myoblast differentiation.
- Gene11007 was identified as a significantly downregulated lncRNA during differentiation.
- Gene11007 was demonstrated to promote buffalo myoblast proliferation but not differentiation.
Conclusions:
- This research expands buffalo genome annotations and identifies gene11007 as a key regulator of myoblast proliferation.
- The findings provide a novel molecular target for understanding lncRNA regulation in skeletal muscle development and improving buffalo meat quality.
Related Concept Videos
01:13Formation of Muscle Fibers from Myoblasts
5.1K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.1K
02:39lncRNA - Long Non-coding RNAs
8.8K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.8K
01:21Satellite Stem Cells and Muscular Dystrophy
2.0K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.0K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
02:23Master Transcription Regulators
7.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.0K
03:03mTOR Signaling and Cancer Progression
3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.9K