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Safety Precautions and Operating Procedures in an ABSL-4 Laboratory: 3. Aerobiology

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Validating Autoclave Cycles for Carcass Disposal in Animal Biosafety Level 2/3 Containment Laboratories.

Rebecca McGirr¹, Christopher Sample², Leslee Arwood³

¹Sanofi Pasteur Limited, Toronto, ON, Canada.

Applied Biosafety : Journal of the American Biological Safety Association

|August 29, 2022

Summary

Sterilizing ferret carcasses requires an extended autoclave cycle (240 minutes at 125°C) compared to general biohazardous waste. This validated method ensures effective decontamination of multiple carcasses for safe disposal.

Keywords:

animal biosafety biocontainment biosafety level 3 decontamination sterilization

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Area of Science:

Veterinary Pathology
Infectious Disease Control
Biomedical Waste Management

Background:

Animal carcasses present unique sterilization challenges due to their composition.
Standard autoclave cycles for general biohazardous waste are insufficient for effective ferret carcass decontamination.

Purpose of the Study:

Develop and validate a specific autoclave cycle for infectious animal carcasses.
Ensure safe removal and disposal of animal carcasses from containment suites.
Evaluate different carcass preparation and packaging methods for autoclaving.

Main Methods:

Biological indicators were used to test autoclave efficacy on intact ferret carcasses.
Varying numbers of carcasses (1-4) were autoclaved in biohazard bags within pans.
Autoclave cycle parameters were optimized and validated for carcasses up to 2 kg.

Main Results:

An extended sterilization time of 240 minutes at 125°C was found necessary.
The validated cycle includes 5 prevacuum pulses and 15 psi chamber pressure.
Successful decontamination was achieved for multiple carcasses per load.

Conclusions:

A validated autoclave cycle can effectively sterilize multiple ferret carcasses.
Extended cycle times are crucial for carcass decontamination compared to general waste.
This method ensures safe disposal of infectious animal remains.