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Identification of Potential miRNA-mRNA Regulatory Network in the Development of Oral Cancer

Yun Yang1, Xin Xin2, Ting Xu3

  • 1Department of Stomatology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai 264000, China.

Disease Markers
|August 29, 2022
PubMed
Abstract

Insights

This study uncovers key microRNAs (miRNAs) like miR-15b-5p and miR-21-5p as potential biomarkers for oral cancer. The research establishes a crucial miRNA-mRNA network for understanding oral cancer progression.

Area of Science:

  • Oncology
  • Bioinformatics
  • Genomics

Background:

  • Oral cancer presents significant challenges in modern medicine with limited effective treatments.
  • Understanding the molecular mechanisms driving oral cancer is crucial for developing novel therapeutic strategies.

Purpose of the Study:

  • To investigate the microRNA-messenger RNA (miRNA-mRNA) network in oral cancer development.
  • To identify potential miRNA biomarkers for oral cancer through bioinformatics analysis.

Main Methods:

  • Utilized bioinformatics analysis on oral cancer datasets (GSE28100, GSE23558) from the GEO database.
  • Employed GEO2R, R language, MiRDIP, DAVID, STRING, and Cytoscape for data processing, target prediction, enrichment analysis, and network visualization.
  • Identified differentially expressed genes (DEGs) and constructed miRNA-mRNA interaction networks.

Main Results:

  • Identified 35 DEGs in GSE28100 and 1651 DEGs in GSE23558.
  • Discovered 143 common genes enriched in pathways such as PI3K/AKT and MAPK.
  • Established a comprehensive miRNA-mRNA network implicated in oral cancer progression.

Conclusions:

  • Identified miR-15b-5p, miR-199a-3p, miR-21-5p, miR-424-5p, and miR-454-3p as potential biomarkers for oral cancer.
  • The established miRNA-mRNA network provides insights into the molecular underpinnings of oral cancer.

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