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Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...

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Updated: Aug 30, 2025

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection

Published on: May 2, 2011

Engineered multivalent self-assembled binder protein against SARS-CoV-2 RBD.

Dustin Britton¹, Kamia Punia¹, Farbod Mahmoudinobar^1,2

¹Department of Chemical and Biomolecular Engineering, New York University Tandon School of Engineering, Brooklyn, New York 11201, USA.

Biochemical Engineering Journal

|August 29, 2022

Summary

Researchers engineered ACE-MAP, a novel protein targeting the SARS-CoV-2 spike RBD. This multivalent approach enhances binding affinity for potential diagnostics and therapeutics against the virus.

Keywords:

Antibody-mimic Diagnostic Protein engineering SARS-CoV-2 Therapeutic

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Engineering Antiviral Agents via Surface Plasmon Resonance

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Published on: June 14, 2022

Area of Science:

Biochemistry
Structural Biology
Virology

Background:

The SARS-CoV-2 pandemic necessitates rapid diagnostics and effective treatments.
The interaction between the SARS-CoV-2 spike receptor binding domain (RBD) and the human ACE2 receptor is a key target.
The N-terminal alpha-helix of ACE2 is crucial for this interaction.

Purpose of the Study:

To engineer a novel protein construct for targeting the SARS-CoV-2 RBD.
To leverage multivalency for enhanced binding avidity, overcoming monomeric ligand limitations.
To develop a stable, expressible protein scaffold for potential therapeutic and diagnostic applications.

Main Methods:

Engineered a fusion protein (ACE-MAP) by combining the ACE2 N-terminal helix with the cartilage oligomeric matrix protein (COMP) coiled-coil domain.
Utilized the COMP scaffold to achieve multivalency and enhance binding to the SARS-CoV-2 RBD.
Assessed protein expression, thermal stability, and binding affinity.

Main Results:

ACE-MAP demonstrates improved binding affinity to the SARS-CoV-2 RBD compared to monomeric approaches.
The engineered protein is expressible in *E. coli*, indicating scalability.
ACE-MAP exhibits good thermal stability and a relatively small molecular weight (62 kDa).

Conclusions:

ACE-MAP represents a promising strategy for developing SARS-CoV-2 diagnostics and therapeutics.
The MAP scaffold offers a versatile platform for engineering multivalent binders.
Targeting the ACE2-RBD interaction via engineered proteins is a viable approach for combating SARS-CoV-2.