Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Improving Translational Accuracy

Improving Translational Accuracy

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Association of Epigenetic Trajectory With Development of Clinical Rheumatoid Arthritis in Individuals With Anti-Citrullinated Protein Antibody Positivity: Targeting Immune Responses for Prevention of Rheumatoid Arthritis (TIP-RA).

Arthritis & rheumatology (Hoboken, N.J.)·2026

Same author

Altered fibroblast-like synoviocyte epigenetics is responsible for deficient NUB1 expression in rheumatoid arthritis.

Scientific reports·2026

Same author

Senescent Activated Naive B Cells Promote Anti-Citrullinated Antigen T Cell Responses and the Transition to Clinical Rheumatoid Arthritis.

bioRxiv : the preprint server for biology·2025

Same author

Process evaluation and lessons learned from the formation of a multi-sector partnership: the Healing Experiences of Adversity among Latinos (HEALthy4You).

Frontiers in health services·2025

Same author

Optimization of a synoviocyte-targeted biologic for inflammatory arthritis in combination or bispecific administration with TNF inhibitors.

JCI insight·2025

Same author

Progression to rheumatoid arthritis in at-risk individuals is defined by systemic inflammation and by T and B cell dysregulation.

Science translational medicine·2025

Same journal

Family cohesion and adaptability in heart failure: an APIMeM analysis of symptom perception and spousal caregiving on patient self-care.

Frontiers in medicine·2026

Same journal

Prognostic factors analysis of surgical resection after conversion therapy for isolated pleural metastatic lung cancer: a retrospective analysis.

Frontiers in medicine·2026

Same journal

Case Report: a novel non-canonical splice site variant in COL4A5 in a patient with Alport syndrome.

Frontiers in medicine·2026

Same journal

Minimally invasive percutaneous cannulated screw fixation for pelvic fractures: a retrospective case cohort study of clinical and radiological outcomes.

Frontiers in medicine·2026

Same journal

A case analysis of Gitelman syndrome complicated with Sjögren's disease.

Frontiers in medicine·2026

Same journal

Inflammatory phenotyping by latent class analysis and machine learning-based prediction of postoperative complications in pediatric appendicitis: a retrospective cohort study.

Frontiers in medicine·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 30, 2025

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Published on: July 22, 2021

Improving Transcriptome Fidelity Following Synovial Tissue Disaggregation.

David L Boyle¹, Edward B Prideaux², Joshua Hillman¹

¹Department of Medicine, University of California, San Diego, San Diego, CA, United States.

Frontiers in Medicine

|August 29, 2022

Summary

This summary is machine-generated.

Optimizing tissue disaggregation for single-cell RNA sequencing (scRNAseq) is crucial. New methods using RNA polymerase inhibitors or cold enzymes better preserve the synovial transcriptome, improving arthritis research.

Keywords:

disaggregation functional genomics rheumatoid arthritis synovia transcriptome

More Related Videos

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

Related Experiment Videos

Last Updated: Aug 30, 2025

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Published on: July 22, 2021

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

Area of Science:

Molecular Biology
Genomics
Immunology

Background:

Synovial tissue analysis is vital for understanding osteoarthritis (OA) and rheumatoid arthritis (RA).
Current disaggregation methods can alter gene expression, affecting transcriptome fidelity.
Single-cell RNA sequencing (scRNAseq) requires high-quality, representative cellular data.

Purpose of the Study:

To enhance the accuracy of the synovial tissue transcriptome after disaggregation.
To evaluate modified disaggregation techniques for improved RNA sequencing applications.
To optimize tissue processing for better differentiation between OA and RA transcriptomes.

Main Methods:

Synovial tissues from OA and RA patients were disaggregated using three methods: Liberase TL (Lib), Lib with flavopridol (Flavo), or cold subtilisin A (SubA).
RNA was extracted and analyzed using quantitative PCR (qPCR) and bulk RNA sequencing (RNAseq).
Results were compared against intact tissue to assess disaggregation-induced changes.

Main Results:

All methods yielded comparable cell yield and viability, with SubA showing improved viability.
Liberase TL alone significantly altered gene expression related to inflammation and immunity.
Flavo and SubA methods minimized disaggregation-induced transcriptome changes, preserving in situ representation.
RNAseq confirmed that alternative methods largely prevented transcriptional alterations.

Conclusions:

Tissue disaggregation significantly impacts the synovial transcriptome.
Employing RNA polymerase inhibitors or cold enzyme digestion preserves transcriptomic integrity.
Optimized disaggregation techniques improve the fidelity of cell suspensions for scRNAseq and other analyses.
Method selection should align with specific research goals for accurate synovial tissue analysis.