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Updated: Aug 30, 2025
Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
Published on: May 25, 2011
Derivation and culturing of neural stem cells from human embryonic brain tissue
Yujin Suk1, Agata Kieliszek2, Daniel Mobilio2
1Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Michael G DeGroote School of Medicine, McMaster University, Hamilton, ON L8S 4L8, Canada.
Abstract:
Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs in vitro from human embryonic brain tissue. We describe the steps to dissociate embryonic brain tissue and culture hNSCs, followed by the procedure to expand hNSCs. These cells can be used for downstream applications including RNA-seq and omics studies. For complete details on the use and execution of this protocol, please refer to Venugopal et al. (2012b), Bakhshinyan et al. (2019), and Venugopal et al. (2012a).
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