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Updated: Aug 30, 2025

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
Published on: July 14, 2017
ACOT7 protects epidermal stem cells against lipid peroxidation
Guang Zhang1,2, Jiaxu Ma1,2, Zhenjie Wu1,2
1Department of Plastic Surgery, Shandong Provincial Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, People's Republic of China.
Acyl-CoA thioesterase 7 (ACOT7) protects epidermal stem cells (ESCs) from oxidative stress by inhibiting lipid peroxidation. This finding is crucial for understanding skin regeneration and repair mechanisms.
Area of Science:
- Dermatology
- Cell Biology
- Biochemistry
Background:
- Epidermal stem cells (ESCs) are vital for skin regeneration.
- Oxidative stress induces lipid peroxidation, impairing skin repair.
- Mechanisms of ESC resistance to lipid peroxidation require further investigation.
Purpose of the Study:
- To investigate the role of Acyl-CoA thioesterase 7 (ACOT7) in epidermal stem cell (ESC) resistance to lipid peroxidation.
- To elucidate the protective effects of ACOT7 against oxidative damage in ESCs.
Main Methods:
- Proteomics analysis to identify proteins correlated with transferrin concentration.
- Overexpression of ACOT7 in ESCs using adenovirus vectors.
- Assessment of lipid peroxidation, cell viability, and malondialdehyde (MDA) levels.
Main Results:
- ACOT7 expression positively correlated with transferrin levels.
- ACOT7 overexpression inhibited lipid peroxidation induced by H₂O₂, Erastin, and RSL3.
- ACOT7 reduced MDA levels and enhanced ESC survival under oxidative stress.
Conclusions:
- ACOT7 plays a protective role against iron-dependent lipid peroxidation in epidermal stem cells.
- ACOT7 enhances ESCs' resilience to oxidative damage, supporting skin repair.
- Targeting ACOT7 may offer therapeutic strategies for skin regeneration.
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