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3D Bioprinted Hydrogel Microfluidic Devices for Parallel Drug Screening.

Anant Bhusal1, Elvan Dogan2, Daniel Nieto3

  • 1Department of Mechanical Engineering, Rowan University, 201 Mullica Hill Road, Glassboro, New Jersey 08028, United States.

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Summary

This study introduces a novel 3D bioprinted microfluidic chip for high-throughput screening (HTS). The innovative design reduces reagent use and costs, enabling efficient in vitro organoid research.

Keywords:
bioprintingdigital light processinghigh-throughput screeninghydrogelmicrofluidicsorgan-on-a-chip

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Area of Science:

  • Biotechnology
  • Materials Science
  • Chemical Engineering

Background:

  • Conventional high-throughput screening (HTS) platforms require large cell volumes, high reagent consumption, and significant assembly costs.
  • Three-dimensional (3D) bioprinted hydrogel-based microfluidic chips offer a potential solution to these limitations.

Purpose of the Study:

  • To develop a continuous and seamless manufacturing approach for 3D bioprinted microfluidic chips.
  • To create a scalable circular-patterned chip for HTS applications.
  • To enable the creation of predefined biochemical gradients within the chip.

Main Methods:

  • Utilized digital light processing 3D bioprinting to fabricate chips from polyethylene glycol diacrylate and cell-laden gelatin methacryloyl.
  • Tuned local chip permeability to control gradient formation.
  • Assessed flow-induced physical characteristics, mass transport of drug agents, and cellular responses (reactive oxygen species).

Main Results:

  • Demonstrated a continuous and seamless manufacturing process for 3D bioprinted microfluidic chips.
  • Successfully created predefined biochemical gradients within the hydrogel-based chip.
  • Validated the chip's capacity for drug/agent gradient delivery and measurement of cellular responses.

Conclusions:

  • The developed hydrogel-based microfluidic chip offers a scalable and cost-effective alternative for HTS.
  • This technology can be adapted for advanced applications such as in vitro organoids.
  • The tunable permeability and gradient generation capabilities are key innovations for future screening platforms.