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Updated: Jul 31, 2026

Primary Culture of Adult Rat Heart Myocytes
Published on: June 16, 2009
[Binding of Ca2+ to glycoprotein from bovine heart mitochondria]
Abstract:
It is shown that glycoprotein from bovine heart mitochondria which forms Ca2+-selective conductance channels in a bilayer lipid membrane possesses Ca2+-binding activity. Ca2+-binding sites of two kinds were revealed in the glycoprotein molecule: high affinity sites with Kd = 2.8 X 10(-6) M and low affinity sites with Kd 1.1 X 10(-5) M. Ca2+-binding by the high affinity sites occurs co-operatively. The Hill coefficient is about 2.
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