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Axonal Regeneration Through Autologous Grafts: Does the Axonal Load Influence Regeneration?
Jonathan I Leckenby1, Miranda A Chacon2, David Milek2
1The Royal Free NHS Foundation Trust, Pond Street, London, United Kingdom; Division of Surgery and Interventional Science, University College London, Gower Street, London, United Kingdom; University of Rochester Medical Center, Rochester, New York.
Introduction:
Two-stage free functional muscle transfers for long-standing facial palsy can yield unpredictable results. Earlier studies have demonstrated incomplete regeneration across neurorrhaphies in native nerve and higher donor axonal counts correlating with improved outcomes but axonal count in nerve grafts have not been as thoroughly reviewed. To investigate the impact of varying axonal counts in autologous grafts on functional outcomes of repair.
Materials And Methods:
Animals were allocated into three groups: Direct Nerve Repair (DNR, n = 50), Small Nerve Graft (SNG, n = 50), and Large Nerve Graft (LNG, n = 50). All grafts were inset into the Posterior Auricular Nerve with ear movement recovery (EMR) monitored as functional outcome. At various postoperative weeks (POWs), excised specimens were imaged with electron microscopy. Axonal counts were measured proximal to, distal (DAC) to, and within grafts. Total Success Ratio (TSR) was calculated.
Results:
In DNR, DAC was significantly lower than proximal axonal counts at all POWs, with maximum TSR of 80%. TSR for LNG and SNG were significantly lower at all POWs when compared to DNR, with maximums of 56% and 38%, respectively. LNG had a significantly larger DAC than SNG at POW12 and beyond. A direct relationship was present between DAC and EMR for all values.
Conclusions:
Higher native axonal count of autologous nerve grafts resulted in higher percentage of regeneration across neurorrhaphies.
