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SP1/miR-92a-1-5p/SOCS5: A novel regulatory axis in feline panleukopenia virus replication

Ruiying Liang1, Lin Liang1, Jingjie Zhao1

  • 1Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

Veterinary Microbiology
|August 29, 2022
PubMed

Insights

Feline panleukopenia virus (FPV) infection alters cellular miR-92a-1-5p expression. This microRNA enhances interferon production, inhibiting FPV replication and boosting host defense.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • MicroRNAs (miRNAs) regulate gene expression and are involved in host-pathogen interactions.
  • Feline panleukopenia virus (FPV) is known to alter host cell miRNA levels, but the precise mechanisms are unclear.
  • Understanding miRNA roles in FPV infection is crucial for developing antiviral strategies.

Purpose of the Study:

  • To investigate the role of specific microRNAs in the host response to FPV infection.
  • To elucidate the molecular mechanisms by which miRNAs modulate FPV replication.
  • To identify potential therapeutic targets for FPV infection.

Main Methods:

  • FPV infection of F81 cells.
  • Quantification of miR-92a-1-5p expression.
  • Analysis of specificity protein 1 (SP1) and suppressor of cytokine signaling 5 (SOCS5) expression.
  • Measurement of interferon (IFN-α/β) and NF-κB signaling pathway activity.

Main Results:

  • FPV infection significantly altered cellular miR-92a-1-5p expression in F81 cells.
  • FPV upregulated SP1, which in turn affected miR-92a-1-5p expression.
  • miR-92a-1-5p enhanced IFN-α/β expression by targeting SOCS5, thereby inhibiting FPV replication.
  • miR-92a-1-5p negatively regulated NF-κB signaling, crucial for host defense.

Conclusions:

  • miR-92a-1-5p plays a significant role in the host defense against FPV infection.
  • The miR-92a-1-5p/SP1/SOCS5/NF-κB axis is a key pathway in modulating FPV replication.
  • Targeting miR-92a-1-5p may offer a novel therapeutic strategy against FPV.
Keywords:
FPVMiR-92a-1–5pSOCS5SP1Type I interferons

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