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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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A Polyaniline-based Sensor of Nucleic Acids
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Red-emitting polyaniline-based nanoparticle probe for pH-sensitive fluorescence imaging.

Lokesh Yadav1, Anjali Yadav2, Shovon Chatterjee3

  • 1Department of Sustainable Energy Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India.

Biomaterials Advances
|August 29, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed water-soluble fluorescent polyaniline nanoparticles (FPNs) for bioimaging. These FPNs offer bright, pH-sensitive red fluorescence and excellent biocompatibility, overcoming limitations of traditional polyaniline nanoparticles.

Keywords:
BioimagingFluorescenceNanoparticlesPhotoluminescence mechanismSulfonated polyaniline

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Semiconducting polymer nanoparticles (NPs) like polyaniline (PANI) typically need external fluorophores for fluorescence.
  • PANI-based NPs face limitations in bioimaging due to weak blue fluorescence and aggregation-caused quenching in physiological conditions.

Purpose of the Study:

  • To develop a facile synthesis method for fluorescent polyaniline nanoparticles (FPNs).
  • To evaluate FPNs as water-soluble, high-intensity, pH-sensitive fluorescent nanoprobes for bioimaging and sensing applications.

Main Methods:

  • Facile solid-state synthesis of fluorescent polyaniline nanoparticles (FPNs).
  • Characterization of FPNs' photoluminescence properties, including pH dependence and quantum yield.
  • Assessment of FPNs' biocompatibility using fibroblast and cancer cell lines.
  • In vitro cellular uptake studies using fluorescence microscopy.

Main Results:

  • FPNs exhibit water-solubility, high intensity, and pH-sensitive red fluorescence with a photoluminescence quantum yield (PLQY) of 19.3% at physiological pH.
  • Exciton-polaron quenching was observed at low pH, with a significant change in emission intensity ratio (600 nm/650 nm) across a wide pH range (2.6 to 11.8).
  • FPNs demonstrated excellent biocompatibility (>85% cell viability) and concentration-dependent cellular uptake in prostate cancer cells.

Conclusions:

  • The developed FPNs are promising fluorescent nanoprobes for bioimaging and ratiometric pH sensing.
  • The facile synthesis and favorable properties of FPNs address limitations of traditional PANI-based NPs.
  • FPNs show potential for applications in biological and medical diagnostics.