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Updated: Aug 30, 2025

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
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Cryptic binding properties of a transient folding intermediate in a PDZ tandem repeat

Francesca Malagrinò1, Giuliana Fusco2, Valeria Pennacchietti1

  • 1Istituto Pasteur - Fondazione Cenci Bolognetti, Dipartimento di Scienze Biochimiche "A. Rossi Fanelli" and Istituto di Biologia e Patologia Molecolari del CNR, Sapienza Università di Roma, Rome, Italy.

Protein Science : a Publication of the Protein Society
|August 30, 2022
PubMed
Summary

This study reveals that a misfolded intermediate of sPDZD2 protein binds its ligand with higher affinity than the native state, suggesting a cryptic function for this folding intermediate.

Keywords:
kineticsmisfoldingprotein domainprotein-protein recognition

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