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Large Animal Model for Evaluating the Efficacy of the Gene Therapy in Ischemic Heart
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Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques.

Spyros A Mavropoulos1, Kelly P Yamada1, Tomoki Sakata1

  • 1Cardiovascular Research Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 30, 2022
PubMed
Summary

Improving cardiac gene delivery via percutaneous antegrade coronary injection requires enhanced techniques. This chapter details improved catheter-based methods in a preclinical model to boost transduction efficiency for better cardiac gene therapy.

Keywords:
Balloon occlusionCardiovascular diseasesGene deliveryGene therapyHomogenous distributionImpellaIntracoronaryMinimally invasiveVectors

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Area of Science:

  • Cardiovascular medicine
  • Gene therapy
  • Interventional cardiology

Background:

  • Percutaneous antegrade coronary injection is a minimally invasive method for cardiac gene delivery.
  • Simple bolus injection shows low transduction efficiency.
  • Enhancement strategies are crucial for effective antegrade intracoronary delivery.

Purpose of the Study:

  • To describe key elements of intracoronary gene delivery.
  • To present protocols for catheter-based antegrade gene delivery techniques.
  • To detail modifications for enhanced transduction efficacy.

Main Methods:

  • Utilized a preclinical large animal model.
  • Developed and refined three distinct catheter-based antegrade gene delivery techniques.
  • Incorporated modifications to improve delivery efficiency.

Main Results:

  • Established protocols for catheter-based antegrade gene delivery.
  • Demonstrated improved transduction efficiency through refined techniques.
  • Provided a comprehensive guide for advanced intracoronary gene delivery.

Conclusions:

  • Catheter-based antegrade gene delivery can be optimized for enhanced cardiac transduction.
  • Refined techniques offer improved efficiency over simple bolus injections.
  • This chapter provides valuable protocols for preclinical research in cardiac gene therapy.