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Related Concept Videos

Gene Therapy00:59

Gene Therapy

25.8K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
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Coronary Artery Disease I: Introduction01:30

Coronary Artery Disease I: Introduction

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Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
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Coronary Artery Disease II: Pathophysiology01:26

Coronary Artery Disease II: Pathophysiology

28
Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
28
Coronary Artery Disease IV: Preventive Measures01:26

Coronary Artery Disease IV: Preventive Measures

31
Effective preventive measures for coronary artery disease (CAD) focus on controlling modifiable risk factors, including cholesterol abnormalities and lifestyle changes.Cholesterol ManagementFirst, the Mediterranean diet and the American Heart Association advocate for maintaining low-density lipoprotein (LDL) cholesterol levels below 100 mg/dL, with a more stringent recommendation of below 70 mg/dL for individuals at high risk. LDL cholesterol, often termed "bad cholesterol," can lead to the...
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Related Experiment Video

Updated: Aug 30, 2025

Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers
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Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers

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Stent-Based Gene Delivery for Coronary Disease.

Ivan S Alferiev1,2, Michael Chorny1,2, Robert L Wilensky2

  • 1The Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 30, 2022
PubMed
Summary
This summary is machine-generated.

This study explores using gene therapy delivered via coronary stents to prevent common complications like in-stent restenosis. Researchers developed a method for attaching viral vectors to stents, showing promising results in early testing.

Keywords:
Adeno-associated virusAdenovirusFluorescent microscopyGene therapyGreen fluorescent proteinIn-stent restenosisLuciferaseStentsSubstrate-mediated gene transfer

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Area of Science:

  • Cardiovascular Medicine
  • Gene Therapy
  • Biomaterials Engineering

Background:

  • Percutaneous coronary interventions (PCI) are standard treatments for advanced coronary artery disease, frequently involving stent placement.
  • Delayed complications such as in-stent restenosis (ISR), late stent thrombosis (LST), and neoatherosclerosis (NA) significantly limit the long-term success of PCI.
  • Current strategies for managing these complications are limited, necessitating novel therapeutic approaches.

Purpose of the Study:

  • To introduce and detail a novel method for utilizing coronary stents as a platform for gene therapy delivery.
  • To mitigate ISR, LST, and NA, a triad of significant delayed complications following PCI.
  • To provide a reproducible methodology for attaching viral vectors to stent surfaces.

Main Methods:

  • Development of a coatless, reversible attachment technique for viral vectors onto metal stent struts.
  • Utilized adenoviral (Ad) and adeno-associated viral (AAV) vectors for gene delivery.
  • In vitro and in vivo studies were conducted to evaluate the efficacy and safety of the gene-eluting stent system.

Main Results:

  • Successful coatless and reversible attachment of Ad and AAV vectors to stent struts was achieved.
  • Demonstrated the feasibility of using gene-eluting stents in relevant vascular models.
  • Preliminary in vitro and in vivo data indicate the potential of this approach to address ISR, LST, and NA.

Conclusions:

  • Coronary stents can be effectively engineered as platforms for localized gene therapy delivery.
  • The developed attachment method offers a promising strategy for mitigating major stent-related complications.
  • This innovative approach holds potential for improving long-term outcomes of PCI.