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Effects of storage on the pyruvate-lactate system and random migration of human granulocytes
Vox Sanguinis
|January 1, 1987
Abstract:
This work demonstrates a time-dependent loss of polymorphonuclear neutrophilic leukocyte (PMN) functions during the first 48 h with only slight lysis and release of cellular LDH. It confirms that storage decreases ATP levels in PMNs and significantly increases intracellular lactate and pyruvate. These data correlated with an inhibition of PMN migratory function and a considerable decrease in chemiluminescence response.
Insights
Storage impairs polymorphonuclear neutrophilic leukocyte (PMN) function within 48 hours, reducing migration and chemiluminescence. This is linked to decreased ATP and increased lactate/pyruvate levels in stored PMNs.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Polymorphonuclear neutrophilic leukocytes (PMNs) are critical immune cells.
- Understanding PMN function during storage is vital for transfusion medicine and research.
Purpose of the Study:
- To investigate the time-dependent changes in PMN function and metabolic state during storage.
- To correlate metabolic shifts with functional impairments in PMNs.
Main Methods:
- Assessing PMN viability via LDH release.
- Measuring intracellular ATP, lactate, and pyruvate levels.
- Evaluating PMN migration and chemiluminescence responses.
Main Results:
- A time-dependent decline in PMN function was observed within 48 hours.
- Storage led to decreased ATP levels and increased intracellular lactate and pyruvate.
- Impaired PMN migratory capacity and reduced chemiluminescence response were noted.
Conclusions:
- Short-term storage significantly compromises PMN function.
- Metabolic alterations, specifically reduced ATP and increased lactate/pyruvate, underlie PMN dysfunction during storage.