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Effect of mirasol pathogen reduction technology system on immunomodulatory molecules of apheresis platelets
S Valsami1, E Grouzi2, D Mochandreou2
1Hematology Laboratory-Blood Bank, Aretaieion Hospital, National and Kapodistrian University of Athens, Athens, Greece.
Abstract:
Pathogen inactivation for platelets by riboflavin system (MIRASOL) efficiently reduces transfusion related pathogen transmission. However little is known about its impact on platelets' immunomodulatory biochemical profile. We aimed was to assess the effects of MIRASOL treatment on platelet quality parameters and immunomodulatory molecules CD62P, RANTES, and CD40L in Single Donor Platelets (SDPs) resuspended in plasma (SDP-P) or T-PAS and additive solution (SDP-A). Twenty nine SDPs (15 SDP-P and 14 SDP-A) were included in the study. Samples were collected before, after MIRASOL treatment and just before transfusion. P-selectin (CD62P), RANTES, and CD40L were tested by ELISA. Platelet products quality assays were also performed. Platelet count/unit decreased after Mirasol treatment by 13 %. The pH of all units decreased over the 5-day storage period but remained above expected limits and the swirling test was positive throughout storage. P-selectin levels were not different between the three different time points in both SDPs-P and SDPs-A while RANTES levels were found to differ statistically significantly at the three different time points in all units and in the SPD-A subgroup. CD40L levels in all SDP products increased slightly during storage but this was not statistically significant. CD62P, RANTES, and CD40L in all time points were elevated in SDPs-A compared to SDPs-P but not at a statistically significant level. In conclusion MIRASOL treatment apart from RANTES increase does not seem to substantially affect platelets associated other cytokines and immunomodulatory molecules namely P-selectin and sCD40L which are implicated in immune transfusion reactions.
Insights
The MIRASOL pathogen inactivation system for platelets shows minimal impact on key immunomodulatory molecules like P-selectin and CD40L, with only RANTES levels significantly affected. This suggests MIRASOL preserves platelet function for transfusion safety.
Area of Science:
- Transfusion Medicine
- Immunology
- Biochemistry
Background:
- The MIRASOL (Pathogen inactivation for platelets by riboflavin system) process effectively reduces pathogen transmission risks associated with platelet transfusions.
- Limited data exists on MIRASOL's impact on the immunomodulatory profile of platelets, crucial for understanding transfusion reactions.
Purpose of the Study:
- To evaluate the effects of MIRASOL treatment on platelet quality and specific immunomodulatory molecules (CD62P, RANTES, CD40L).
- To compare these effects in Single Donor Platelets (SDPs) stored in plasma (SDP-P) versus an additive solution (SDP-A).
Main Methods:
- Twenty-nine SDP units (15 SDP-P, 14 SDP-A) were analyzed before MIRASOL treatment, after treatment, and before transfusion.
- Levels of P-selectin (CD62P), RANTES, and CD40L were quantified using ELISA.
- Standard platelet quality assays, including pH and swirling tests, were performed.
Main Results:
- Platelet count decreased by 13% post-MIRASOL treatment.
- RANTES levels showed statistically significant changes over time in all units and the SDP-A subgroup.
- P-selectin and CD40L levels remained largely unaffected by MIRASOL treatment, although RANTES increased.
- SDPs stored in additive solution (SDP-A) exhibited higher levels of CD62P, RANTES, and CD40L compared to SDPs in plasma (SDP-P).
Conclusions:
- MIRASOL treatment, apart from a RANTES increase, does not significantly alter platelet levels of P-selectin and sCD40L.
- These findings suggest that MIRASOL maintains the immunomodulatory profile of platelets, potentially minimizing immune transfusion reactions.
- Platelet storage in additive solutions may influence baseline levels of certain immunomodulatory molecules compared to plasma storage.
