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Related Experiment Video

Updated: Aug 30, 2025

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Published on: January 7, 2012

Cellular Respiration in Thymic Fragments from Mice.

Mariam Alshamsi¹, Maya Hassane¹, Farida Almarzooqi²

¹Department of Microbiology and Immunology, College of Medicine and Health Sciences, UAE University, 15551 Al Ain, Abu Dhabi, United Arab Emirates.

Frontiers in Bioscience (Landmark Edition)

|August 30, 2022

Summary

Phosphorescence oxygen analysis measures thymocyte respiration in vitro. Sirolimus and ozanimod, common immunosuppressants, significantly reduced oxygen consumption in thymic fragments, indicating their impact on cellular metabolism.

Keywords:

cellular bioenergetics cellular respiration immunosuppressants immunotoxins mTOR ozanimod sirolimus thymus

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Isolation and Transplantation of Different Aged Murine Thymic Grafts.

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Published on: May 13, 2015

Area of Science:

Immunology
Pharmacology
Biochemistry

Background:

Investigating in vitro thymic responses to pharmaceutical agents is crucial for understanding immune modulation.
Sirolimus (mTOR inhibitor) and ozanimod (S1P receptor agonist) are key immunomodulatory drugs with distinct mechanisms of action.

Purpose of the Study:

To detail the use of phosphorescence oxygen analyzer for in vitro thymic response assessment.
To evaluate the impact of sirolimus and ozanimod on thymocyte respiration.

Main Methods:

In vitro incubation of mouse thymic fragments in oxygen-sensing medium.
Monitoring cellular oxygen consumption over time using phosphorescence oxygen analyzer.
Quantifying thymocyte respiration rate (μM O2 min-1 mg-1) and assessing inhibition by pharmaceutical agents.

Main Results:

Thymocyte respiration rate was linear and confirmed to be cytochrome oxidase-dependent.
Baseline thymocyte respiration rate in control mice was established (0.046 ± 0.011 μM O2 min-1 mg-1).
Sirolimus inhibited respiration by 16%, and ozanimod by 29% in thymic fragments.

Conclusions:

Thymocyte respiration serves as a valid surrogate biomarker for evaluating drug effects.
This method aids in studying the mode-of-action and cytotoxicity of immunosuppressants and immunomodulators.
Phosphorescence oxygen analysis offers a sensitive tool for in vitro drug screening in immunology.