Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

866
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
866

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Test-retest reliability of brain responses to risk-taking during the balloon analogue risk task.

NeuroImage·2019
Same author

Prdx6 is required to protect human corneal epithelial cells against ultraviolet B injury.

European journal of ophthalmology·2019
Same author

Structure of the Cardiac Sodium Channel.

Cell·2019
Same author

The clinicopathologic features and prognosis of esophageal neuroendocrine carcinomas: a single-center study of 53 resection cases.

BMC cancer·2019
Same author

LAMTOR5 raises abnormal initiation of O-glycosylation in breast cancer metastasis via modulating GALNT1 activity.

Oncogene·2019
Same author

Mucoadhesive dexamethasone-glycol chitosan nanoparticles for ophthalmic drug delivery.

International journal of pharmaceutics·2019

Related Experiment Video

Updated: Aug 30, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.6K

Universal Markers for hiPSCs Residue Detection.

Hui Shi1, Tiejun Feng2, Rui Wang2

  • 1Molecular Pharmacology Laboratory, Institute of Molecular Medicine, Peking University, 100871 Beijing, China.

Frontiers in Bioscience (Landmark Edition)
|August 30, 2022
PubMed
Summary

A universal marker for residual undifferentiated induced pluripotent stem cells (iPSCs) is crucial for cell therapy. Researchers identified ESRG and ZSCAN10 as sensitive, quantitative markers for iPSC detection, benefiting quality control.

Keywords:
cell therapyddPCRhuman pluripotent stem cell

More Related Videos

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
07:19

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

Published on: February 1, 2011

15.1K
GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
11:06

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

Published on: May 19, 2012

17.1K

Related Experiment Videos

Last Updated: Aug 30, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

22.6K
High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
07:19

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue

Published on: February 1, 2011

15.1K
GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
11:06

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

Published on: May 19, 2012

17.1K

Area of Science:

  • Stem Cell Biology
  • Molecular Biology
  • Genomics

Background:

  • Detecting residual undifferentiated induced pluripotent stem cells (iPSCs) is vital for the safety of cell therapy products derived from embryonic stem cells (ESCs) and iPSCs.
  • Current methods for residual detection are gene-specific, costly, and time-consuming, highlighting the need for a universal marker applicable across all germline cell types.

Purpose of the Study:

  • To identify and validate a universal marker for detecting residual undifferentiated iPSCs in cell therapy products.
  • To develop a sensitive and quantitative method for quality control in pluripotent stem cell (PSC)-derived therapies.

Main Methods:

  • Utilized next-generation sequencing (NGS) to analyze gene expression in iPSCs and ESCs, identifying candidate marker genes.
  • Cross-referenced candidate genes with the Genotype-Tissue Expression (GTEx) database to assess tissue expression patterns.
  • Employed droplet digital PCR (ddPCR) for sensitive detection and quantification of residual iPSCs using the identified marker genes.

Main Results:

  • Identified ESRG and ZSCAN10 as two highly expressed genes in iPSCs with minimal background expression in differentiated cells.
  • Confirmed broad applicability and high sensitivity of ESRG/ZSCAN10 detection, capable of identifying as low as 0.0001% residual undifferentiated hiPSCs.
  • Demonstrated that ESRG and ZSCAN10 transcripts serve as effective universal markers for undifferentiated iPSC detection.

Conclusions:

  • ESRG and ZSCAN10 are validated as sensitive and quantitative universal markers for residual undifferentiated iPSC detection.
  • The ddPCR method targeting ESRG/ZSCAN10 transcripts offers a broadly applicable quality control solution for iPSC-derived cell therapies.
  • This discovery significantly advances the safety and efficacy of stem cell-based therapeutic applications.