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Related Experiment Videos

An improved isolated working rabbit heart preparation using red cell enhanced perfusate.

V Chen, Y H Chen, S E Downing

    The Yale Journal of Biology and Medicine
    |May 1, 1987
    PubMed
    Summary

    Adding red blood cells (RBCs) to the perfusate significantly improved the mechanical and metabolic stability of isolated working rabbit hearts. This enhancement is attributed to an adequate oxygen supply, crucial for cardiac function.

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    Area of Science:

    • Cardiovascular Physiology
    • Organ Perfusion Studies

    Background:

    • Isolated working heart models are crucial for studying cardiac function.
    • Oxygen supply is a critical factor in maintaining cardiac performance and metabolic stability.

    Purpose of the Study:

    • To compare the performance of isolated working rabbit hearts perfused with Krebs-Henseleit (KH) buffer versus KH buffer supplemented with human red blood cells (RBCs).
    • To evaluate the impact of red blood cells on cardiac mechanical function and metabolic stability.

    Main Methods:

    • Isolated working rabbit hearts were perfused with either KH buffer or KH buffer with 15% hematocrit of washed human RBCs.
    • Cardiac performance metrics including coronary flow, aortic flow, peak systolic pressure, and stroke work were measured.
    • Oxygen consumption, ATP, glycogen, and triacylglycerol content were analyzed.

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    Main Results:

    • Hearts perfused with KH + RBC exhibited higher aortic flow and peak systolic pressure compared to KH alone.
    • Oxygen uptake was significantly lower and less responsive to filling pressures in hearts perfused with KH alone.
    • Hearts perfused with KH + RBC demonstrated stable performance for at least 150 minutes, unlike those with KH alone which showed reduced ATP and glycogen.

    Conclusions:

    • Supplementation of the perfusate with red blood cells significantly enhances the mechanical and metabolic stability of isolated working rabbit hearts.
    • Adequate oxygen supply, facilitated by RBCs, is essential for sustaining cardiac function in vitro.
    • The inclusion of RBCs in the perfusate is vital for long-term studies of cardiac physiology in isolated heart models.