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Updated: Aug 30, 2025
Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells
Published on: July 25, 2016
Protocol to derive human trophoblast stem cells directly from primed pluripotent stem cells
Yanxing Wei1, Lu Xiao2, Lishi Ma2
1Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5T3H7, Canada; Key Laboratory of Functional Proteomics of Guangdong Province, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China; The First Clinical Medical School, Southern Medical University, Guangzhou 510515, China.
None:
Human trophoblast stem cells (hTSCs) are useful for studying human placenta development and diseases, but primed human pluripotent stem cells (hPSCs) routinely cultured in most laboratories do not support hTSC derivation. Here, we present a protocol to derive hTSCs directly from primed hPSCs. This approach, containing two strategies either with or without bone morphogenetic protein 4 (BMP4), provides a simple and accessible tool for deriving hTSCs to study placenta development and disease modeling without ethical limitations or reprogramming process. For complete details on the use and execution of this protocol, please refer to Wei et al. (2021).
