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CircCLTH promotes skeletal muscle development and regeneration
Mengjie Chen1, Qingyou Liu1,2, Mingming Song1
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.
Epigenetics
|August 31, 2022
Summary
Researchers identified circCLTH, a novel circular RNA that enhances buffalo skeletal muscle development. This circular RNA (circRNA) promotes myoblast proliferation and differentiation, aiding muscle regeneration.
Area of Science:
- Animal Science
- Molecular Biology
- Genetics
Background:
- Buffalo possess significant potential for beef production.
- Circular RNAs (circRNAs) are crucial regulators of myogenesis, but their role in buffalo skeletal muscle development remains unclear.
Purpose of the Study:
- To investigate the expression profiles and regulatory functions of circRNAs during buffalo myoblast proliferation and differentiation.
- To identify novel circRNAs involved in buffalo skeletal muscle development.
Main Methods:
- RNA sequencing (RNA-seq) was employed to analyze circRNA expression profiles in buffalo myoblasts.
- Bioinformatic analysis identified differentially expressed circRNAs.
- Cellular experiments and RNA pull-down assays were conducted to elucidate the function of a specific circRNA, circCLTH.
- In vivo overexpression studies were performed to assess circCLTH's role in skeletal muscle regeneration.
Main Results:
- A total of 3,142 circRNA candidates were identified, with 110 found to be differentially expressed between proliferation and differentiation stages.
- A novel circRNA, circCLTH (347 nt), specifically expressed in muscle tissue and highly conserved across species, was characterized.
- circCLTH was found to interact with PLEC protein, promoting myoblast proliferation and differentiation while inhibiting apoptosis.
- In vivo overexpression of circCLTH stimulated skeletal muscle regeneration.
Conclusions:
- circCLTH is a novel, conserved non-coding RNA regulator that significantly promotes buffalo myoblast proliferation and differentiation.
- circCLTH plays a vital role in skeletal muscle development and regeneration in buffalo.
- This finding provides new insights into the molecular mechanisms governing myogenesis in livestock.
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